Transfection of mammalian cells with DNA vectors that can replicate autonomously as plasmids is advantageous for several types of experiments. Plasmids that replicate to high copy number provide amplification of signals for DNA, RNA, and protein synthesis. Also, plasmid DNA can be easily retrieved from mammalian cells, separate from the mass of chromosomal DNA. Thus, the use of autonomously replicating recombinant DNA vectors, in conjunction with transfection techniques for transfer of the vectors to mammalian cells, has produced rapid and powerful experiments that avoid many of the complexities associated with the full-sized mammalian genome.
KeywordsMutation Frequency Shuttle Vector Monkey Cell Mutant Plasmid High Mutation Frequency
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