The stable introduction of cloned genes into mammalian cells has proven to be a widely used method to study the DNA sequences responsible for the regulation of eukaryotic gene expression. An implicit assumption of this approach is that transfected genes are capable of expression in the foreign cell and that transcription occurs at levels high enough to detect. There are now abundant examples of tissue-specific genes introduced into mammalian cells that are transcribed at measurable levels. This chapter will review examples of cell-specific genes and developmentally regulated genes that are expressed in foreign cellular environments and examine the parameters that govern the expression of these genes in mammalian cells. A generality that emerges from these examples is that cloned genes that are introduced into cells are frequently expressed even though the endogenous counterpart genes are not. Furthermore, several genes expressed normally in a tissue-specific manner can be regulated appropriately even when they are present in a foreign cellular environment.
KeywordsGlobin Gene Mouse Mammary Tumor Virus Immunoglobulin Gene Thymidine Kinase Gene Globulin Gene
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