Microcell Fusion and Mammalian Gene Transfer
Microcell-mediated chromosome transfer (Fournier and Ruddle, 1977a; Fournier, 1981) is a parasexual genetic technique that can be used to transfer single, intact chromosomes from one mammalian cell to another. As such, microcell fusion is not a gene-transfer method in the usual sense of the term; rather, it is an approach for the construction of simple hybrid cells with precisely defined genotypes. Microcell hybrids have proved to be valuable tools for mammalian gene mapping, for genetic analyses of complex cellular phenotypes, and, in conjunction with other gene-transfer methods, for genetic manipulation of specific chromosomes and chromosomal regions.
KeywordsMouse Chromosome Recipient Cell Hybrid Clone Tyrosine Aminotransferase Donor Chromosome
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