Intrachromosomal Recombination in Mammalian Cells
The technologies of gene transfer and recombinant DNA can be exploited to construct artificial gene duplications of a selectable gene in cultured mammalian cells. These pairs of closely linked genes, harboring different mutations, are stably integrated in the host genome by means of a separate dominant selectable marker and therefore provide an opportunity to systematically study the process of homologous recombination as it occurs between repeated chromosomal sequences in cultured mammalian cells.
KeywordsHomologous Recombination Parent Line Recombination Event Herpes Simplex Virus Type Inverted Repeat
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