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Gene Transfer: A Perspective

  • Frank H. Ruddle

Abstract

Somatic-cell genetics is a large, multifaceted field that is not easily defined. This book addresses one subfield of this unruly subject—that concerned with para-sexuality. Tissue culture had its origins in the experiments of Ross Harrison (1907) early in this century, but a consideration of sexuality in cultured cell populations did not arise until the 1950s. This way of thinking was motivated by the demonstration of parasexuality in eukaryotes, particularly by Stern (1936) and Pontecorvo (1962), and by sexuality in prokaryotes, as demonstrated by Lederberg (1958). In a sense, the work of Stern dealing with mitotic recombi­nation was the more relevant because it dealt with somatic cells, albeit in vivo. However, the demonstration of sexuality in free-living bacteria was more dra­matic and, although only analogous, powerfully influenced the development of parasexual systems in cultured prokaryotic cells in vitro.

Keywords

Thymidine Kinase Thymidine Kinase Gene Culture Cell Population Herpes Thymidine Kinase Herpes Virus Thymidine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bacchetti, S., and Graham, F., 1977, Transfer of the gene for thymidine kinase to thymidine kinase deficient human cells by means of purified herpes simplex viral DNA, Proc. Natl. Acad. Sci. U.S.A. 74: 1590–1594.PubMedCrossRefGoogle Scholar
  2. Barski, G., Sorieul, S., and Cornefert, F., 1960, Production dans des cultures in vitro de deux souches cellulaires en association de cellules de caractere “hybride,” C. R. Acad. Sci. 251: 1825–1830.Google Scholar
  3. Caspersson, T., Farber, S., Foley, G. E., Kudynoski, J., Modest, E. J., Simonsson, E., Wagh, U., and Zeck, L., 1968, Chemical differentiation along metaphase chromosomes, Exp. Cell Res. 49: 219–222.PubMedCrossRefGoogle Scholar
  4. Chilton, M.-D., Drummond, M. H., Merlo, D. J., Sciaky, D., Montoya, A., Gordon, M. P., and Nester, E. W., 1977, Stable incorporation of plasmid DNA into higher plant cells: The molecular basis of crown gall tumorigenesis, Cell 11: 263–271.PubMedCrossRefGoogle Scholar
  5. Ephrussi, B., and Weiss, M. C., 1965, Interspecific hybridization of somatic cells, Proc. Natl. Acad. Sci. U.S.A. 53: 1040–1042.PubMedCrossRefGoogle Scholar
  6. Fournier, R. E. K., and Ruddle, F. H., 1977, Microcell mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells, Proc. Natl. Acad. Sci. U.S.A. 74: 319–323.PubMedCrossRefGoogle Scholar
  7. Gordon, J. W., Scangos, G., Plotkin, D., Barbosa, J., and Ruddle, F. H., 1980, Genetic transformation of mouse embryos by microinjection of purified DNA, Proc. Natl. Acad. Sci. U.S.A. 77: 7380–7384.PubMedCrossRefGoogle Scholar
  8. Graham, F. L., and van der Eb, A. J., 1973, A new technique for the assay of infectivity of human adenovirus 5 DNA, Virology 521: 456–467.CrossRefGoogle Scholar
  9. Harris, H., and Watkins, J. F., 1965, Hybrid cells derived from mouse and man: Artificial heterokaryons of mammalian cells from different species, Nature (London) 205: 640–646.CrossRefGoogle Scholar
  10. Harris, M., and Ruddle, F. H., 1961, Clone strains of pig kidney cells with drug resistance and chromosomal markers, J. Natl. Cancer Inst. 26: 1405–1411.PubMedGoogle Scholar
  11. Harrison, R. G., 1907, Observations on the living developing nerve fiber, Anat. Rec. 1: 116–118.CrossRefGoogle Scholar
  12. Hinnen, A., Hicks, J. B., and Fink, G. R., 1978, Transformation of yeast, Proc. Natl. Acad. Sci. U.S.A. 75: 1929–1933.PubMedCrossRefGoogle Scholar
  13. Lederberg, J., 1958, Genetic approaches to somatic cell variation: Summary comment, J. Cell. Physiol. 52: 383–401.CrossRefGoogle Scholar
  14. Littlefield, J., 1964, Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants, Science 145: 709–710.PubMedCrossRefGoogle Scholar
  15. Maitland, H., and McDougall, J., 1977, Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA, Cell 11: 233–241.PubMedCrossRefGoogle Scholar
  16. McBride, O. W., and Ozer, H. L., 1973, Transfer of genetic information by purified metaphase chromosomes, Proc. Natl. Acad. Sci. U.S.A. 70: 1258–1262.PubMedCrossRefGoogle Scholar
  17. Munyon, Y., Kraiselburd, E., Davis, D., and Mann, J., 1971, Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus, J. Virol. 7: 813–820.PubMedGoogle Scholar
  18. Okada, Y., 1958, The fusion of Ehrlich’s tumor cells caused by HVJ virus in vitro., Biken J. 1: 103–110.Google Scholar
  19. Pontecorvo, G., 1962, Methods in microbiological genetics in an approach to human genetics, Br. Med. Bull. 18: 18–84.Google Scholar
  20. Puck, T. T., 1972, The Mammalian Cell as a Microorganism: Genetic and Biochemical Studies in Vitro, Holden-Day, San Francisco, 1972.Google Scholar
  21. Rubin, G. M., and Spradling, A. C., 1982, Genetic transformation of Drosophila using transposable element vectors, Science 218: 348–353.PubMedCrossRefGoogle Scholar
  22. Ruddle, F. H., 1968, Isozymic variants as genetic markers in somatic cell populations in vitro, Natl. Cancer Inst. Monogr. 29: 9–13.Google Scholar
  23. Smiley, J. R., Steege, D. A., Juricek, D. K., Summers, W., and Ruddle, F. H., 1978, Herpes simplex virus 1 integration site in the mouse genome defined by somatic cell genetic analysis, Cell 15: 455–468.PubMedCrossRefGoogle Scholar
  24. Smithies, O., Gregg, R. G., Boggs, S. S., Koralewski, M. A., and Kucherlapati, R. S., 1985, Insertion of DNA sequences into the human chromosomal 3-globin locus by homologous recombination, Nature (London) 317: 230–234.CrossRefGoogle Scholar
  25. Stem, C., 1936, Somatic crossing over and segregation in Drosophila melanogaster, Genetics 21: 625–730.Google Scholar
  26. Szybalska, E. H., and Szybalski, W., 1962, Genetics of human cell lines. IV. DNA mediated heritable transformation of a biochemical trait, Proc. Natl. Acad. Sci. U.S.A. 48: 2026–2034.PubMedCrossRefGoogle Scholar
  27. Weiss, M. C., and Green, H., 1967, Human—mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes, Proc. Natl. Acad. Sci. U.S.A. 58: 1104–1111.PubMedCrossRefGoogle Scholar
  28. Wigler, M., Silverstein S., Lee, L.-S., Pellicer, A., Cheng, T., and Axel, R., 1977, Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells, Cell 11: 223–232.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Frank H. Ruddle
    • 1
  1. 1.Department of BiologyYale UniversityNew HavenUSA

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