Gene Transfer: A Perspective

  • Frank H. Ruddle


Somatic-cell genetics is a large, multifaceted field that is not easily defined. This book addresses one subfield of this unruly subject—that concerned with para-sexuality. Tissue culture had its origins in the experiments of Ross Harrison (1907) early in this century, but a consideration of sexuality in cultured cell populations did not arise until the 1950s. This way of thinking was motivated by the demonstration of parasexuality in eukaryotes, particularly by Stern (1936) and Pontecorvo (1962), and by sexuality in prokaryotes, as demonstrated by Lederberg (1958). In a sense, the work of Stern dealing with mitotic recombi­nation was the more relevant because it dealt with somatic cells, albeit in vivo. However, the demonstration of sexuality in free-living bacteria was more dra­matic and, although only analogous, powerfully influenced the development of parasexual systems in cultured prokaryotic cells in vitro.


Thymidine Kinase Thymidine Kinase Gene Culture Cell Population Herpes Thymidine Kinase Herpes Virus Thymidine 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Frank H. Ruddle
    • 1
  1. 1.Department of BiologyYale UniversityNew HavenUSA

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