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Structure and Expression of IgE Receptors

  • Anthony KulczyckiJr.

Abstract

Cell surface receptors with high affinity for IgE are present only on mast cells, basophils, and neoplastic counterparts of these cells. Monomeric IgE is bound to these receptors and displayed on the mast cell/basophil surface. Subsequently, multivalent antigen may bind to adjacent receptor-bound IgE molecules. The primary role of these IgE receptors appears to be transducing this cross-linking signal in order to initiate mediator release. The specificity of these IgE receptors (or Fcε receptors) on basophils for IgE was first shown in studies with human peripheral blood leukocytes (Ishizaka et al., 1970; Sullivan et al., 1971). More detailed studies of the structure, function, and expression of the high-affinity Fcε receptors have been made possible by use of the rat basophilic leukemia (RBL) cell line (Kulczycki et al., 1974).

Keywords

Mast Cell Human Basophil Human Peripheral Blood Leukocyte Multivalent Antigen Human Macrophage Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anderson, C. L., and Spiegelberg, H. L., 1981, Macrophage receptors for IgE: Binding of IgE to specific IgE Fc receptors on a human macrophage cell line, U937, J. Immunol. 126: 2470.PubMedGoogle Scholar
  2. Becker, K. E., Ishizaka, T., Metzger, H., Ishizaka, K., and Grimley, P., 1973, Surface IgE on human basophils during histamine release, J. Exp. Med. 138: 394.PubMedCrossRefGoogle Scholar
  3. Boltz-Nitulescu, G., Plummer, J. M., and Spiegelberg, H. L., 1982, Fc receptors for IgE on mouse macrophages and macrophage-like cell lines, J. Immunol. 128: 2265.PubMedGoogle Scholar
  4. Brown, M. S., Anderson, R. G. W., and Goldstein, J. L., 1983, Recycling receptors: The round-trip itinerary of migrant membrane proteins, Cell 32: 663.PubMedCrossRefGoogle Scholar
  5. Capron, A., Dessaint, J.-P., Capron, M., and Bazin, H., 1975, Specific IgE antibodies in immune adherence of normal macrophages to Schistosoma mansoni schistosomules, Nature (London) 253: 474.CrossRefGoogle Scholar
  6. Capron, M., Capron, A., Dessaint, J.-P., Torpier, G., Johansson, S. G. O., and Prin, L., 1981a, Fc receptors for IgE on human and rat eosinophils, J. Immunol. 126: 2087.PubMedGoogle Scholar
  7. Capron, M., Bazin, H., Joseph, M., and Capron, A., 1981b, Evidence for IgE-dependent cytotoxicity by rat eosinophils, J. Immunol. 126: 1764.PubMedGoogle Scholar
  8. Carson, D. A., and Metzger, H., 1974, Interaction of IgE with rat basophilic leukemia cells. IV. Antibody-induced redistribution of IgE receptors, J. Immunol 113: 1271.PubMedGoogle Scholar
  9. Conrad, D. H., and Froese, A., 1976, Characterization of the target cell receptor for IgE. II. Poly-acrylamide gel analysis of the surface IgE receptor from normal rat mast cells and from rat basophilic leukemia cells, J. Immunol. 116: 319.PubMedGoogle Scholar
  10. Conrad, D. H., Bazin, H., Sehon, A. H., and Froese, A., 1975, Binding parameters of the interaction between rat IgE and rat mast cell receptors, J. Immunol. 114: 1688.PubMedGoogle Scholar
  11. Conrad, D. H., Wingard, J. R., and Ishizaka, T., 1983, The interaction of human and rodent IgE with the human basophil IgE receptor, J. Immunol. 130: 327.PubMedGoogle Scholar
  12. Farah, F. S., Kern, M., and Eisen, H., 1960, Specific inhibition of wheal-and-erythema responses with univalent haptens and univalent antibody fragments, J. Exp. Med. 112: 1211.PubMedCrossRefGoogle Scholar
  13. Fewtrell, C., Goetze, A., and Metzger, H., 1982, Phosphorylation of the receptor for IgE, Biochemistry 21: 2004.Google Scholar
  14. Finbloom, D. S., and Metzger, H., 1982, Binding of immunoglobulin E to the receptor on rat peritoneal macrophages, J. Immunol. 129: 2004.Google Scholar
  15. Finbloom, D. S., and Metzger, H., 1983, Isolation of cross-linked IgE-receptor complexes from rat macrophages, J. Immunol. 130: 1489.PubMedGoogle Scholar
  16. Goetze, A., Kanellopoulos, J., Rice, D., and Metzger, H., 1981, Enzymatic cleavage products of the a subunit of the receptor for IgE, Biochemistry 20: 6341.PubMedCrossRefGoogle Scholar
  17. Gonzalez-Molina, A., and Spiegelberg, H. L., 1977, A subpopulation of normal human peripheral B lymphocytes that bind IgE, J. Clin. Invest. 59: 616.PubMedCrossRefGoogle Scholar
  18. Hempstead, B. L., Parker, C. W., and Kulczycki, A., Jr., 1979, Characterization of the IgE receptor isolated from human basophils, J. Immunol. 123: 2283.PubMedGoogle Scholar
  19. Hempstead, B. L., Parker, C. W., and Kulczycki, A., Jr., 1981a, The cell surface receptor for IgE: Effect of tunicamycin on molecular properties of receptor from rat basophilic leukemia cells, J. Biol. Chem. 256: 10717.PubMedGoogle Scholar
  20. Hempstead, B. L., Kulczycki, A., Jr., and Parker, C. W., 1981b, Phosphorylation of the IgE receptor from ionophore A23187 stimulated intact rat mast cells, Biochem. Biophys. Res. Commun. 98: 815.CrossRefGoogle Scholar
  21. Hempstead, B. L., Parker, C. W., and Kulczycki, A., Jr., 1983, Selective phosphorylation of IgE receptor in antigen-stimulated rat mast cells, Proc. Natl. Acad. Sci. USA 80: 3050.PubMedCrossRefGoogle Scholar
  22. Hirashima, M., Yodoi, J., and Ishizaka, K., 1980, Regulatory role of IgE-binding factors from rat T lymphocytes. III. IgE-specific suppressive factor with IgE-binding activity, J. Immunol. 125: 1442.PubMedGoogle Scholar
  23. Holowka, D., and Baird, B., 1983, Structural studies on the membrane-bound IgE-receptor complex. 2. Mapping of distances between sites on IgE and the membrane surface, Biochemistry 22: 3475.CrossRefGoogle Scholar
  24. Holowka, D., Hartmann, H., Kanellopoulos, J., and Metzger, H., 1980, Association of the receptor for IgE with an endogenous polypeptide on rat basophilic leukemia cells, J. Receptor Res. 1: 41.Google Scholar
  25. Holowka, D., Gitler, C., Bertovici, T., and Metzger, H., 1981, Reaction of 5-iodonaphthyl-1-nitrene with the IgE receptor on normal and tumor mast cells, Nature (London) 289: 806.CrossRefGoogle Scholar
  26. Isersky, C., Metzger, H., and Buell, D. N., 1975, Cell cycle-associated changes in receptors for IgE during growth and differentiation of a rat basophilic leukemia cell line, J. Exp. Med. 141: 1147.PubMedCrossRefGoogle Scholar
  27. Isersky, C., Taurog, J. D., Poy, G., and Metzger, H., 1978, Triggering of cultured neoplastic mast cells by antibodies to the receptor for IgE, J. Immunol. 121: 549.PubMedGoogle Scholar
  28. Isersky, C., Rivera, J., Mims, S., and Triche, T. J., 1979, The fate of IgE bound to rat basophilic leukemia cells, J. Immunol. 122: 1926.PubMedGoogle Scholar
  29. Isersky, C., Rivera, J., Segal, D. M., and Triche, T., 1983, The fate of IgE bound to rat basophilic leukemia cells. II. Endocytosis of IgE oligomers and effect on receptor turnover, J. Immunol. 131: 388.PubMedGoogle Scholar
  30. Ishizaka, K., and Ishizaka, T., 1969, Immune mechanisms of reversed type reaginic hypersensitivity, J. Immunol. 103: 588.PubMedGoogle Scholar
  31. Ishizaka, K., Tomioka, H., and Ishizaka, T., 1970, Mechanisms of passive sensitization. I. Presence of IgE and IgG molecules of human leukocytes, J. Immunol. 105: 1459.PubMedGoogle Scholar
  32. Ishizaka, T., Soto, C., and Ishizaka, K., 1973, Mechanisms of passive sensitization. III. Number of IgE molecules and its receptor sites on human basophil granulocytes, J. Immunol. 111: 500.PubMedGoogle Scholar
  33. Ishizaka, T., Ishizaka, K., Conrad, D. H., and Froese, A., 1978, A new concept of triggering mechanisms of IgE-mediated histamine release, J. Allergy Clin. Immunol. 61: 320.PubMedCrossRefGoogle Scholar
  34. Kanellopoulos, J. M., Liu, T.-Y., Poy, G., and Metzger, H., 1980, Composition and subunit structure of the cell receptor for IgE, J. Biol. Chem. 255: 9060.PubMedGoogle Scholar
  35. Kepron, M. R., Conrad, D. H., and Froese, A., 1982, The cross-reactivity of rat IgE and IgG with solubilized receptors of rat basophilic leukemia cells, Mol. Immunol. 19: 1631.Google Scholar
  36. Kulczycki, A., Jr., 1981, Role of IgE and IgE receptors in bronchial asthma, J. Allergy Clin. Immunol. 68: 5.PubMedCrossRefGoogle Scholar
  37. Kulczycki, A., and Metzger, H., 1974, The interaction of IgE with rat basophilic leukemia cells. II. Quantitative aspects of the binding reaction, J. Exp. Med. 140: 1676.PubMedCrossRefGoogle Scholar
  38. Kulczycki, A., and Parker, C. W., 1979, The cell-surface receptor for IgE. I. The use of repetitive affinity chromatography for the purification of a mammalian receptor, J. Biol. Chem. 254: 3187.Google Scholar
  39. Kulczycki, A., Isersky, C., and Metzger, H., 1974, The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE, J. Exp. Med. 139: 600.Google Scholar
  40. Kulczycki, A., McNearney, T. A., and Parker, C. W., 1976, The rat basophilic leukemia cell receptor for IgE. I. Characterization as a glycoprotein, J. Immunol 117: 661.PubMedGoogle Scholar
  41. Kumar, N., and Metzger, H., 1982, Gel filtration in 6 M guanidine HC1 of the a-subunit of the receptor for IgE, Mol. Immunol. 19: 1561.Google Scholar
  42. Melewicz, F. M., Plummer, J. M., and Spiegelberg, H. L., 1982, Comparison of the Fc receptors for IgE on human lymphocytes and monocytes, J. Immunol. 129: 563.PubMedGoogle Scholar
  43. Metzger, H., Alcaraz, G., Hohman, R., Kinet, J.-P., Pribluda, V., and Quarto, R., 1986, The receptor with high affinity for IgE, Ann. Rev. Immunol. 4: 419.CrossRefGoogle Scholar
  44. Parker, C. W., Schechtel, T., Falkenhein, S., and Huber, M., 1983, Induction of IgE receptors on human lymphocytes, Immunol. Commun. 12: 1.Google Scholar
  45. Perez-Montfort, R., and Metzger, H., 1982, Proteolysis of soluble IgE receptor complexes: Localization of sites on IgE which interact with the Fc receptor, Mol. Immunol. 19: 1113.Google Scholar
  46. Siraganian, R. P., Hook, W. A., and Levine, B. B., 1975, Histamine release from basophils: Activation by bivalent haptens, Immunochemistry 12: 149.CrossRefGoogle Scholar
  47. Spiegelberg, H. L., 1983, Fc receptors for IgE on macrophages and lymphocytes: IgE synthesis, Fed. Proc. 42: 122.Google Scholar
  48. Spiegelberg, H. L., and Simon, R. A., 1981, Increase of lymphocytes with Fc receptors for IgE in patients with allergic rhinitis during the grass pollen season, J. Clin. Invest. 68: 845.PubMedCrossRefGoogle Scholar
  49. Suemura, J., Yodoi, J., Hirashima, M., and Ishizaka, K., 1980, Regulatory role of IgE-binding factors from rat T lymphocytes. I. Mechanisms of enhancement of IgE response by IgE-potentiating factor, J. Immunol. 125: 148.PubMedGoogle Scholar
  50. Sullivan, A. L., Grimley, P. M., and Metzger, H., 1971, Electron microscopic localization of IgE on the surface membrane of human basophils, J. Exp. Med. 134: 1403.PubMedCrossRefGoogle Scholar
  51. Yodoi, J., and Ishizaka, K., 1979, Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fce receptors, J. Immunol. 122: 2577.PubMedGoogle Scholar
  52. Yodoi, J., Ishizaka, T., and Ishizaka, K., 1979, Lymphocytes bearing Fc receptors for IgE. II. Induction of Fce-receptor bearing rat lymphocytes by IgE, J. Immunol. 123: 455.PubMedGoogle Scholar
  53. Yodoi, J., Hirashima, M., and Ishizaka, K., 1981, Lymphocytes bearing Fc receptors for IgE. V. Effect of tunicamycin on the formation of IgE-potentiating factor and IgE-suppressive factor by Con A-activated lymphocytes, J. Immunol. 126: 877.PubMedGoogle Scholar
  54. Yodoi, J., Hirashima, M., and Ishizaka, K., 1982, Regulatory role of IgE-binding factors from rat T lymphocytes. V. The carbohydrate moieties in IgE-potentiating factors and IgE-suppressive factors, J. Immunol. 128: 289.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Anthony KulczyckiJr.
    • 1
  1. 1.Department of Internal Medicine, Division of Allergy and ImmunologyWashington University School of MedicineSt. LouisUSA

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