Amniotic Fluid Cell Culture
This chapter is intended as a supplement and an update, rather than a replacement, of the chapter on this subject in the previous edition of the work (Milunsky, 1979), and the reader is encouraged to peruse that chapter along with the present one. Much valuable information deriving from the extensive personal experience of the author of that chapter (Milunsky, 1973, 1979) has remained accurate and timely, and thus will not be repeated here. This also holds for the comprehensive list of references to the early work in the field. The present chapter will deal mainly with areas in which significant recent progress has been made. One substantial advance concerns the identification and classification of colony-forming amniotic fluid cell types. Another relates to the profound impact of culture medium supplements on the success and speed of amniotic fluid cell growth.
KeywordsAmniotic Fluid Prenatal Diagnosis Cloning Efficiency Amniotic Fluid Cell Chromosomal Mosaicism
Unable to display preview. Download preview PDF.
- Boué, J., Nicolas, H., Barichard, F., et al., 1979, Le clonage des cellules du liquide amniotique aide dans l’interprétation des mosaiques chromosomiques en diagnostic prénatal, Ann. Genet. 22:3.Google Scholar
- Burton, B. K., Gerbie, A. B., and Nadler, H. L., 1979, Biochemical and biological problems and pitfalls of cell culture for prenatal diagnosis, in: Genetic Disorders and the Fetus (A. Milunsky, ed.), p. 369, Plenum Press, New York.Google Scholar
- Chang, H. C., and Jones, O. W., 1982, Amniocentesis: Cell culture of human amniotic fluid in a hormone supplement, in: Cold Spring Harbor Conferences on Cell Proliferation, Vol. 9, p. 1187, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
- Epstein, C. J., 1982, The use of growth factors to stimulate the proliferation of amniotic fluid cells, Meth. Cell Biol. 26:264.Google Scholar
- Harrison, H., Martin, A. O., Gemme, M. A., et al., 1983, Analysis of amniotic fluid supernatant and cellular proteins with high resolution two-dimensional electrophoresis, Am. J. Hum. Genet. 35:45A.Google Scholar
- Huisjes, H. J., 1978, Cytology of the amniotic fluid and its clinical applications, in: Amniotic Fluid, Research and Clinical Application (D. V. I. Fairweather and T. K. A. B. Eskes, eds.), 2nd ed., p. 93, Elsevier/North-Holland, Amsterdam.Google Scholar
- Melnyk, J. H., Persinger, G., and Teplitz, R. L. A., 1979, A micromethod for processing amniotic fluid cells, In Vitro 15:200.Google Scholar
- Milunsky, A., 1973, The Prenatal Diagnosis of Hereditary Disorders, Thomas, Springfield, Illinois.Google Scholar
- Milunsky, A., 1979. Amniotic fluid cell culture, in: Genetic Disorders and the Fetus (A. Milunsky, ed.), p. 75, Plenum Press, New York.Google Scholar
- Salk, D. S., Disteche, C., Stenchever, M. R., et al., 1983, Routine use of Chang medium for prenatal diagnosis: Improved growth and increased chromosome breakage, Am. J. Hum. Genet. 35:151 A.Google Scholar
- Therkelsen, A. J., 1979, Cell culture and cytogenetic techniques, in: Prenatal Diagnosis (J. D. Murken, S. Stengel-Rutkowski, and E. Schwinger, eds.) p. 258, Enke, Stuttgart.Google Scholar
- Uhlendorf, B.W., 1970, Use of amniotic fluid, and reliability of diagnostic procedures, in: Early diagnosis of human genetic defects. Fogarty Int. Proc. 6:149–162.Google Scholar