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Human Metaphase Chromosomes

Analysis and Sorting by Flow Cytometry
  • D. K. Green
  • Judith A. Fantes
  • H. J. Evans

Abstract

Flow cytometry (Melamed et al., 1979) has provided the cytogeneticist with a fast and accurate method for measuring the quantity of DNA in each human chromosome and, more significantly perhaps, a method for purifying chromosomes that have similar amounts of DNA. Analysis rates are usually in excess of 1000 chromosomes/sec, and following the accumulation of fluorescence intensity data from, say 105 chromosomes, which typically takes less than 2 min, it is claimed that chromosome DNA can be estimated to an accuracy of one band, which is 1/600th of the genome (Langlois et al., 1982). Isolating chromosomes in the same group takes somewhat longer. When metaphase chromosomes are passed through a flow cytometer at a rate of 1000/sec, 106 homologous chromosomes from one group will be accumulated over a period of 6 hr.

Keywords

Human Chromosome Metaphase Chromosome Duchenne Muscular Dystrophy Fluorescence Profile Light Scatter Intensity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Aubrey Milunsky 1986

Authors and Affiliations

  • D. K. Green
    • 1
  • Judith A. Fantes
    • 1
  • H. J. Evans
    • 1
  1. 1.Medical Research Council, Clinical and Population Cytogenetics UnitWestern General HospitalEdinburghScotland

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