Rat Islet Endocrine Cells Contain Metand Leu-Enkephalins in High- and Low-Molecular-Weight Forms
The rate of insulin secretion is known to be influenced by a large number of substances, including glucose and other substrates, ions, other hormones, and neurotransmitters. For example, Lerner and Porte (1971) showed that an epinephrine infusion profoundly inhibited the acute, first-phase insulin secretory response to glucose in normal humans. More recently, following the reports by Margules et al. (1978) and others (Rossier et al., 1979) of increased β-endorphin in pituitary of genetically obese hyperinsulinemic mice, evidence has begun to accumulate that suggests a role for endogenous opioid peptides in the modulation of insulin secretion and possibly in the pathophysiology of the diabetic state. Types of evidence include pharmacological studies of opiate effects on islet function, a smaller number of reports of the presence in pancreas of endogenous opioids, and a few reports suggesting that islets may secrete endogenous opioid peptides.
KeywordsInsulin Secretion Islet Cell Insulin Release Opioid Peptide Opiate Receptor
Unable to display preview. Download preview PDF.
- Actams, J. C., 1981, Heavy metal intensification of DAB-based HRP reaction product, J. Histochem. Cytochem. 29:775.Google Scholar
- Barkey, R. J., Würzburger, R. J., and Spector, S., 1981, Selective opiate binding sites in the rat pancreas, Pharmacologist 23:380.Google Scholar
- Jeanrenaud, X., Maeder, E., Del Pozo, E., and Felber, J. P., 1981, Enkephalin-induced glucose-insulin dissociation in man, Diabetologia 21:287.Google Scholar
- Kilpatrick, D. L., Taniguchi, T., Jones, B. N., Stern, A. S., Shively, J. E., Hullihan, J., Kimura, S., Stein, S., and Udenfriend, S., 1981, A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]-and [Leu]enkephalin sequence, Proc. Natl. Acad. Sci. U.S.A. 78:3265–3268.PubMedCrossRefGoogle Scholar
- Mizuno, K., Minamino, N., Kangawa, K., and Matsuo, H., 1980, A new family of endogenous “big” met-enkephalins from bovine adrenal medulla: Purification and structure of docosa-(BAM-22P) and eicosapeptide (BAM-20P) with very potent opiate activity, Biochem. Biophys. Res. Commun. 97:1283–1290.PubMedCrossRefGoogle Scholar
- Morley, J. E., Baranetsky, N. G., Wingert, T. D., Carlson, H. E., Hershman, J. M., Melmed, S., Levin, S. R., Jamison, K. R., Weitzman, R., Chang, R. J., and Varner, A. A., 1980, Endocrine effects of naloxone induced opiate receptor blockade, J. Clin. Endocrinol. Metab. 50:251–257.PubMedCrossRefGoogle Scholar
- Robinson, R. P., Williams, P. E., Gooch, B. R., and Abumrad, N. N., 1982, The effect of morphine and naloxone on plasma glucagon and glucose production in the diabetic dog. Diabetes (Suppl. 2)31:161A.Google Scholar
- Rossier, J., Liston, D., Patey, G., Chaminade, M., Foutz, A. S., Cupo, A., Giraud, P., Roisin, M. P., Henry, J. P., Verbanck, P., and Vanderhaeghen, J. J., 1983, The enkephalinergic neuron: Implications of a polyenkephalin precursor, Cold Spring Harbor Symp. Quant. Biol. 48:393–404.PubMedCrossRefGoogle Scholar
- Sternberger, L. A., 1974, Immunocytochemistry, Prentice-Hall, Englewood Cliffs, N. J.Google Scholar