The Neuroanatomic Correlates of the Behavioral Effects of Bombesin

  • S. Johnston
  • P. Parmashwar
  • Z. Merali
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Bombesin (BN), a tetradecapeptide initially isolated from anuran skin (Anastasi et al., 1971), has become the standard of reference for comparison of the effects of a series of BN-like peptides recently isolated from several other species including rat, pig, chicken, dog, and human. These BN-like peptides share structural ho-mology, particularly at the carboxyl-terminal heptapeptide region (McDonald et al., 1979, 1980; Reeve et al, 1983; Orloff et al., 1984; Spindel et al. y 1984; Minamino et al., 1984).


Locomotor Activity Nucleus Accumbens Nucleus Tractus Solitarius Behavioral Profile Guide Cannulae 
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  1. Anastasi A., Erspamer, V., and Bucci, M., 1971, Isolation and structure of bombesin and alytesin, two analogous active peptides from the skin of the European amphibians Bombina. Alytes, Experientia 27:166–167.CrossRefGoogle Scholar
  2. Babu, G. N., and Vijayan, E., 1983, Plasma gonadotropin, prolactin levels and hypothalamic tyrosine hydroxylase activity following intraventricular bombesin and secretin in ovariectomized conscious rats, Brain Res. Bull. 11:25–29.PubMedCrossRefGoogle Scholar
  3. Bolles, R. C., 1960, Grooming behavior in the rat, J. Comp. Physiol. Psychol. 53:306–310.PubMedCrossRefGoogle Scholar
  4. Brown, M. R., 1983, Central nervous system sites of action of bombesin and somatostatin to influence plasma epinephrine levels, Brain Res. 276:253–257.PubMedCrossRefGoogle Scholar
  5. Brown, M., and Vale, W., 1980, in: Thermoregulatory Mechanisms and Their Therapeutic Implications (B. Cox, P. Lomax, A. S. Milton, and E. Schonbaum, eds.), S. Karger, Basel, pp. 186–194.Google Scholar
  6. Brown, M., Rivier, J., and Vale, W., 1977a, Actions of bombesin, thyrotropin releasing factor, prostaglandin E and naloxone on thermorégulation in the rat, Life Sci. 20:1681–1688.PubMedCrossRefGoogle Scholar
  7. Brown, M., Rivier, J., and Vale, W., 1977b, Bombesin: Potent effects on thermorégulation in rat, Science 196:998–1000.PubMedCrossRefGoogle Scholar
  8. Brown, M., Rivier, J., and Vale, W., 1977c, Bombesin affects the central nervous system to produce hyperglycemia in rats, Life Sci. 21:1729–1734.PubMedCrossRefGoogle Scholar
  9. Brown, M., Allen, R., Villareal, J., Rivier, J., and Vale, W., 1978, Bombesin-like activity: Radioim-munologic assessment in biological tissues, Life Sci. 23:2721–2728.PubMedCrossRefGoogle Scholar
  10. Brown, M., Tache, Y., and Fisher, D., 1979, Central nervous system action of bombesin: Mechanism to induce hyperglycemia, Endocrinology 105:660–665.PubMedCrossRefGoogle Scholar
  11. Carpenter, M. B., 1985, Core Text of Neuroanatomy, third edition, Williams & Wilkins, Baltimore, pp. 125–127.Google Scholar
  12. Chronister, R. B., Sikes, R. W., Trow, T. W., and DeFrance J. F., 1981, The organization of the nucleus accumbens, in: The Neurobiology of the Nucleus Accumbens (R. B. Chronister and J. F. DeFrance, eds.), Haer Institute, Brunswick, ME, pp. 97–146.Google Scholar
  13. Collu, R., Marchisio, A.-M., and Tache, Y., 1983, Inhibitory action of bombesin on prolactin release induced by dopamine antagonists, Neuroendocrinol. Lett. 5:265–272.Google Scholar
  14. Cowan, A., Khunawat, P., Zu Zhu, X, and Gmerek, D. E. IV, 1985, Effects of bombesin on behaviour, Life Sci. 37:135–145.PubMedCrossRefGoogle Scholar
  15. Crawley, J. N., and Moody, T. W., 1983, Anxiolytics block excessive grooming behaviour induced by ACTH 1-24 and bombesin, Brain Res. Bull. 10:399–401.PubMedCrossRefGoogle Scholar
  16. Crawley, J. N., and Schwaber, J. S., 1983, Nucleus tractus solitarius lesions block the behavioural actions of cholecystokinin, Peptides 4:143–141.Google Scholar
  17. Dockray, G. J., Valliant, C., and Walsh, J. H., 1979, The neuronal origin of bombesin-like immu-noreactivity in the rat gastrointestinal tract, Neuroscience 4:1561–1568.PubMedCrossRefGoogle Scholar
  18. Fink, J. S., and Smith, G. P., 1980, Mesolimbicocortical dopamine terminal fields are necessary for normal locomotor and investigatory exploration in rats, Brain Res. 199:359–384.PubMedCrossRefGoogle Scholar
  19. Fisher, L. A., and Brown, M. R., 1984, Bombesin-induced stimulation of cardiac parasympathetic innervation, Regul. Peptides 8:335–343.CrossRefGoogle Scholar
  20. Folkers, K., Hakanson, R., Horig, J., Jie-Cheng, X., and Leander, S., 1984, Biological evaluation of substance P antagonists, Br. J. Pharmacol. 83:449–456.PubMedGoogle Scholar
  21. Gibbs, J., 1985, Effect of bombesin on feeding, Life Sci. 37:147–153.PubMedCrossRefGoogle Scholar
  22. Gispen, W. H., and Isaacson, R. L., 1981, ACTH-induced excesive grooming in the rat, Pharmacol. Ther. 12:206–246.CrossRefGoogle Scholar
  23. Gmerek, D. E., and Cowan, A., 1983, Studies on bombesin-induced grooming in rats, Peptides 4:907–913.PubMedCrossRefGoogle Scholar
  24. Gmerek, D. E., and Cowan, A., 1984, In vivo evidence for benzomorphan-selective receptors in rats, J. Pharmacol. Exp. Ther. 230:110–115.PubMedGoogle Scholar
  25. Gmerek, D. E., Cowan, A., and Vaught, J. L., 1983, Intrathecal bombesin in rats: Effects on behaviour and gastrointestinal transit, Eur. J. Pharmacol. 94:141–143.PubMedCrossRefGoogle Scholar
  26. Gunion, M., Tache, Y., and Walsh, J. H., 1983, Gastric hyposecretion and hyperglycemia induced by bombesin injection near the paraventricular nucleus, Soc. Neurosci. Abstr. 3:442.Google Scholar
  27. Isaacson, R. L., 1984, Hippocampal damage: Effects on dopaminergic systems of the basal ganglia, in: International Review of Neurobiology, Vol. 25 (J. Smythes and R. Bradley, eds.), Academic Press, New York, pp. 339–359.Google Scholar
  28. Jensen, R. T., Jones, S. W., Folkers, K., and Gardner, J. D., 1984, A synthetic peptide that is a bombesin receptor antagonist, Nature 309:61–63.PubMedCrossRefGoogle Scholar
  29. Katz, R., 1980, Grooming elicited by intraventricular bombesin and eledoisin in the mouse, Neuro-pharmacology 19:143–146.Google Scholar
  30. Kelly, P. H., and Iversen, S. D., 1976, Selective 6-OHDA-induced destruction of mesolimbic dopamine neurons: Abolition of psychostimulant-induced locomotor activity in rats, Eur. J. Pharmacol. 40:45–56.PubMedCrossRefGoogle Scholar
  31. Kirk, R. E., 1982, Experimental Design: Procedures for the Behavioral Sciences, Brooks/Cole, Monterey, CA, p. 115.Google Scholar
  32. Kulkosky, P. J., Gibbs, J., and Smith, G. P., 1982a, Behavioural effects of bombesin administration in rats, Physiol. Behav. 28:505–512.PubMedCrossRefGoogle Scholar
  33. Kulkosky, P. J., Gibbs, J., and Smith, G. P., 1982b, Feeding suppression and grooming repeatedly elicited by intraventricular bombesin, Brain Res. 242:194–196.PubMedCrossRefGoogle Scholar
  34. Lindvall, O., and Bjorklund, A., 1983, Dopamine and norepinephrine-containing neuron systems: Their anatomy in the rat brain, in: Chemical Neuroanatomy (P. C. Emson, ed.), Raven Press, New York, pp. 229–255.Google Scholar
  35. McDonald, T. J., Jornvall, H., Nilsson, G., Vagne, M., Ghatei M., Bloom, S. R., and Mutt, V., 1979, Characterization of a gastrin releasing peptide from porcine non-antral gastric tissue, Biochem. Biophys. Res. Commun. 90:227–233.PubMedCrossRefGoogle Scholar
  36. McDonald, T. J., Jornvall, H., Ghatei, M., Bloom, S. R., and Mutt, V., 1980, Characterization of an avian (proventricular) peptide having sequence homology with porcine gastrin-releasing peptide and the amphibian peptides bombesin and alystensin, FEBS Lett. 122:45–48.PubMedCrossRefGoogle Scholar
  37. Merali, Z., Johnston, S., and Zalcman, S., 1983, Bombesin-induced behavioural changes: Antagonism by neuroleptics, Peptides 4:693–697.PubMedCrossRefGoogle Scholar
  38. Merali, Z., Johnston, S., and Sistek, J., 1985, Role of dopaminergic system(s) in mediation of the behavioural effects of bombesin, Pharmacol. Biochem. Behav. 23:243–248.PubMedCrossRefGoogle Scholar
  39. Minamino, N., Kangawa, K., and Matsuo, H., 1984, Neuromedin B is a major bombesin-like peptide in rat brain: Regional distribution of neuromedin B and neuromedin C in rat brain, pituitary and spinal cord, Biochem. Biophys. Res. Commun. 124:925–932.PubMedCrossRefGoogle Scholar
  40. Moody, T. W., and Pert, C. B., 1979, Bombesin-like peptides in rat brain: Quantitation and biochemical characterization, Biochem. Biophys. Res. Commun. 90:7–14.PubMedCrossRefGoogle Scholar
  41. Moody, T. W., Thoa, N. B., O’Donohue, T. L., and Pert, C. B., 1980, Bombesin-like peptides in rat brain: Localization in synaptosomes and release from hypothalamic slices, Life Sci. 26:1707–1712.PubMedCrossRefGoogle Scholar
  42. Moody, T. W., O’Donohue, T. L., and Jacobowitz, D. M., 1981, Biochemical localization and characterization of bombesin-like peptides in discrete regions of rat brain, Peptides 2:75–79.PubMedCrossRefGoogle Scholar
  43. Moody, T. W., Crawley, J. N., and Jensen, R. T., 1982, Pharmacology and neurochemistry of bombesin-like peptides, Peptides 3:559–563.PubMedCrossRefGoogle Scholar
  44. O’Donohue, T. L., Massari, J., Pazoles, C. J., Chronwall, B. M., Shults, C. W., Quirion, R., Chase, T. N., and Moody, T. W., 1984, A role for bombesin in sensory processing in the spinal cord, J. Neurosci. 412:2956–2962.Google Scholar
  45. Orloff, M. S., Reeve, J. R., Jr., Ben-Avram, C. M., Shively, J. E., and Walsh, J. H., 1984, Isolation and sequence analysis of human bombesin-like peptides, Peptides 5:865–870.PubMedCrossRefGoogle Scholar
  46. Panula, P., Yang, H.-Y. T., and Costa, E., 1982, Neuronal location of the bombesin-like immuno-reactivity in the central nervous system of the rat, Regul. Peptides 4:275–283.CrossRefGoogle Scholar
  47. Parrott, R. F., and Baldwin, B. A., 1982, Centrally-administered bombesin produces effects unlike short term satiety in opérant feeding pigs, Physiol. Behav. 28:521–524.PubMedCrossRefGoogle Scholar
  48. Paxinos, G., and Watson, C., 1982, The Rat Brain in Stereotaxic Coordinates, Academic Press, New York.Google Scholar
  49. Pert, A., Moody, T. W., Pert, C. B., DeWald, L. A., and Rivier, J., 1980, Bombesin: Receptor distribution in brain and effects on nocioception and locomotor activity, Brain Res. 193:209–220.PubMedCrossRefGoogle Scholar
  50. Pittman, Q., Tache, Y., and Brown, M., 1980, Bombesin acts in preoptic areas to produce hypothermia in rats, Life Sci. 26:725–730.PubMedCrossRefGoogle Scholar
  51. Reeve, J. R., Jr., Walsh, J. H., Chew, P., Clark, B., Hawke, D., and Shively, J. E., 1983, Amino acid sequences of three bombesin-like peptides from canine intestine extracts. J. Biol. Chem. 258:5582–5588.PubMedGoogle Scholar
  52. Schulz, D. W., Kalivas, P. W., Nemeroff, C. B., and Prange, A. J., Jr., 1984, Bombesin-induced locomotor hyperactivity: Evaluation of the involvement of mesolimbic dopamine system, Brain Res. 304:377–382.PubMedCrossRefGoogle Scholar
  53. Soveny, C., Mercuri, J., and Hansky, J., 1984, Distribution of bombesin-and cholecystokinin-like immunoreactivity in rat and dog brain and gastrointestinal tract, Regul. Peptides 9:61–68.CrossRefGoogle Scholar
  54. Spindel, E. R., Chin, W. W., Price, J., Rees, L. H., Besser, G. M., and Habener, J. F., 1984, Cloning and characterization of cDNAs encoding human gastrin-releasing peptide, Proc. Natl. Acad. Sci. U.S.A. 81:5699–5703.PubMedCrossRefGoogle Scholar
  55. Spruijt, B., and Gispen, W. H., 1983, ACTH and grooming behaviour in the rat, in: Hormones and Behaviour in Higher Vertebrates (J. Balthazart, E. Prove, and R. Gilles, eds.), Springer-Verlag, Berlin, Heidelberg, pp. 118–136.CrossRefGoogle Scholar
  56. Stuckey, J. A., and Gibbs, J., 1982, Lateral hypothalamic injection of bombesin decreases food intake in rats, Brain Res. Bull. 8:617–621.PubMedCrossRefGoogle Scholar
  57. Tache, Y., 1982, Bombesin: Central nervous system action to increase gastric mucus in rats, Gastro-enterology 83:75–80.Google Scholar
  58. Tache, Y., and Brown, M., 1982, On the role of bombesin in homeostatis, Trends Neurosci. Res. 5:431–433.CrossRefGoogle Scholar
  59. Tache, Y., and Collu, R., 1982, CNS mediated inhibition of gastric secretion by bombesin: Independence from interaction with brain catecholaminergic and serotoninergic pathways and pituitary hormones, Regul. Peptides 3:51–59.CrossRefGoogle Scholar
  60. Tache, Y., Brown, M., and Collu, R., 1979, Effects of neuropeptides on adenohypophyseal hormone response to acute stress in male rats, Endocrinology 105:220–224.PubMedCrossRefGoogle Scholar
  61. Tache, Y., Grijalva, C. V., Gunion, M. W., Cooper, P. H., Walsh J. H., and Novin, D., 1984, Lateral hypothalamic mediation of hypergastrinemia induced by intracisternal bombesin, Neuroendocri-nology 39:114–119.Google Scholar
  62. Widerlov, E., Mueller, R. A., Frye, G. D., and Breese, G. R., 1984, Bombesin increases dopamine function in rat brain areas, Peptides 5:523–528.PubMedCrossRefGoogle Scholar
  63. Wolf, S. S., Moody, T. W., O’Donohue, T. L., Zarbin, M. A., and Kuhar, M. J., 1983, Autoradiographic visualization of rat brain binding sites for bombesin-like peptides, Eur. J. Pharmacol. 87:163–164.PubMedCrossRefGoogle Scholar
  64. Wunder, B. A., Hawkins, M. F., Avery, D. D., and Swan, H., 1980, The effects of bombesin injected into the anterior and posterior hypothalamus on body temperature and oxygen consumption, Neu-ropharmacology 19:1095–1097.Google Scholar
  65. Yachnis, A. T., Crawley, J. N., Jensen, R. T., McGrane, M. W., and Moody, T. W., 1984, The antagonism of bombesin in the CNS by substance P analogues, Life Sci. 35:1963–1969.PubMedCrossRefGoogle Scholar
  66. Zarbin, M. A., Kuhar, M. J., O’Donohue, T. L., Wolf, S. S., and Moody, T. W., 1985, Autoradiographic localization of (125I-Tyr4)bombesin-binding sites in rat brain, J. Neurosci. 5(2):429-437.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • S. Johnston
    • 1
  • P. Parmashwar
    • 1
  • Z. Merali
    • 1
  1. 1.School of PsychologyUniversity of OttawaOttawaCanada

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