Value of Determination of Kininase II in Bronchoalveolar Lavage Fluid
Kininase II (KII), identical with angiotensin-I-converting enzyme (E.C. 220.127.116.11) was characterized biochemically and assayed fluorimetrically in bronchoalveolar lavage fluid and serum of 153 patients with several pulmonary disorders. The albumin concentrations of serum and bronchoalveolar lavage fluid (BLF) have also been measured. The pH optimum of KII derived from BLF (LKII) was 8.0. The Michaelis Menten constant was 38.5 μmol/1 using benzyloxycarbonyl-phenylalanyl-histidyl-leucine as synthetic substrate. LKII could be inhibited between 80 and 100% by EDTA, phenanthroline, dimer- capto-l-propane-sulfonic adic (DMPS), hydroxyquinoline and Captopril. The LKII activity (mU/ml BLF) showed no differences in all lung diseases, but the specific LKII (mU/mg albumin) was significantly elevated in sarcoidosis compared to pneumonia (p < 0.05), fibrosis (p < 0.05), chronic obstructive bronchitis (p < 0.005) and lung cancer (p < 0.01), but not in tuberculosis. This study shows that LKII is measurable in native, unconcentrated BLF and the results indicate that LKII could be useful for diagnosis of pulmonary disorders.
KeywordsAlbumin Concentration Bronchoalveolar Lavage Fluid Alveolar Space Alveolar Cell Synthetic Substrate
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