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Kinins IV pp 487-494 | Cite as

Biological Regulation of Testicular Angiotensin I-Converting Enzyme

  • Paul A. Velletri
  • Walter Lovenberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)

Summary

Angiotensin I-converting enzyme (ACE) from rat testes and lung was purified to homogeneity and partially characterized with respect to physicochemical parameters. Additionally, the biological regulation of testicular ACE by gonadotropins and androgens was investigated and the cell type with which ACE is associated in testes was identified. Rat testicular ACE is a lower molecular weight, isozymic version of the lung enzyme. Partial proteolysis of each isozyme produces different peptide maps, suggesting unique primary structures for each protein. The sensitivity of each isozyme to Co2+, chelators and thermal denaturation is different, a finding that further supports the hypothesis that structural differences exist between the two isozymes. The pituitary gland is essential for the development during puberty and maintenance during adulthood of testicular ACE. In hypophysectomized mature rats, gonadotropins or androgen can maintain ACE activity to near sham-operated levels. ACE activity in testes appears to be associated almost entirely with various stages of germinal cell development. The function(s) of testicular ACE awaits definition. The mechanism of androgen-maintenance of testicular ACE is unclear. Whether androgen specifically induces gene expression of testicular ACE or simply allows for ACE activity to develop in parallel with spermatogenesis is an unresolved question.

Keywords

Pituitary Gland Thermal Denaturation Pubertal Development Germinal Cell Biological Regulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Velletri, P. A., Billingsley, M. L. and Lovenberg, W., Thermal denaturation of rat pulmonary and testicular angiotensin converting enzyme isozymes: effects of chelators and CoCl2, Biochim. Biophys. Acta, submitted.Google Scholar
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    Velletri, P. A., Aquilano, D. Bruckwick, E., Tsai-Morris, C. H., Dufau, M. L. and Lovenberg, W., Endocrinological control and cellular localization of rat testicular angiotensin converting enzyme (E.C. 3.4.15.1), Endocrinology, submitted.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Paul A. Velletri
    • 1
  • Walter Lovenberg
    • 1
  1. 1.Section of Biochemical PharmacologyNational Heart, Lung and Blood InstituteBethesdaUSA

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