Studies on Carbohydrate Structure and Immunological Properties of Human Urinary Kallikreins
Some studies on carbohydrate structure and immunological properties of human urinary kallikreins purified from the human urine of healthy men (HUK) were investigated. A general technique for fractionating asparagine- linked oligosaccharides of HUK was applied. This involves serial chromatographies on concanavalin A-Sepharose 4B and other lectins bound Agarose. Asparagine-linked main oligosaccharides of both active and inactive types of HUK were mixture of tri- or tetra-antennary complex oligosaccharide having GlcNACβ1-4Manβ residue (bisecting GlcNAc) (type I-2), bi-antennary complex oligosaccharide having core Fuc αl-6GlcNAc residue (type III-3), tri- or tetra-antennary complex oligosaccharides having Galβl-4GlcNAcβl-4 (Galβ1-4GlcNAcβ1-2) Man α-residue (type I-l-A-a) etc. Besides these structural studies, the method to probe the heterogenous profiles of HUK due to the different structures of carbohydrate chains bound, was devised without the complete purification of urinary kallikrein even with individual subject of not only active but also inactive forms of the kallikrein.
KeywordsWheat Germ Agglutinin Carbohydrate Moiety Carbohydrate Structure Carbohydrate Chain Tissue Kallikrein
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