Advertisement

Kinins IV pp 355-359 | Cite as

Analytical Study of Kallikrein and Kallikrein-Like Esterase Activity in Subfractions from Rat Kidney Cortex Microsomes and Isolated Subcellular Membranes

  • Knut-Jan Andersen
  • Jarle Ofstad
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)

Summary

Heavy and light microsomal fractions were subfractioned using high performance zonal rotors, and assayed for approtenin sensitive kallikrein- like amidolytic activity (pH 8.2). The activity profiles for the various substrates assayed show rather complex distribution pattern demonstrating kallikrein-like amidolytic activity in plasma membranes, basolateral membranes, rough endoplasmic reticulum and membranes derived from the Golgi complex.

Keywords

Plasma Kallikrein Amidolytic Activity Glandular Kallikrein Zonal Rotor Renal Kallikrein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    P. Friberger, Chromogenic peptide substrates. Their use for the assay of factors in the fibrinolytic and plasma kallikrein-kinin systems, Scand, J. Clin. Lab. Invest., 42(suppl. 162 ): 11–37.Google Scholar
  2. 2.
    O. A. Carretero, A. G. Scicli, and A. Nasjletti, The glandular kallikrein-kinin system: Methodology for its measurement, in: “Hypertension Research - Methods and Models,” F. M. Radzialowski, ed., Marcel Dekker (1982).Google Scholar
  3. 3.
    K. Kimura, Segmental localization of kallikrein-like pro-pheargnaph-thylester in the rat nephron, Acta Path. Microbiol. Immunol. Scand., 91 (Sect. A): 35–42 (1983).Google Scholar
  4. 4.
    K. Kimura and H. Moriya, Enzyme- and immuno-histo-chemical localization of kallikrein. II. The human kidney, Histochemistry, 80: 443–448 (1984).PubMedCrossRefGoogle Scholar
  5. 5.
    Kabi Diagnostics (S-11287 Stockholm, Sweden), Products for diagnostic use and research (1983).Google Scholar
  6. 6.
    K-J. Andersen, H. J. Haga, and M. Dobrota, Heterogeneity of rat kidney-cortex lysosomes fractionated by gradient centrifugation in zonal rotors, Biochem. Soc. Trans., 8: 5976–598 (1980).Google Scholar
  7. 7.
    K-J. Andersen, M. Dobrota, and H. J. Haga, The effect of sucrose on assaying enzymes and protein in the subcellular fractions of the rat kidney cortex, J. Biochem. Biophys. Methods, 1: 309–311 (1979).PubMedCrossRefGoogle Scholar
  8. 8.
    A. Schwartz, K. Nagano, M. Nakao, G. E. Lindenmayer, and J. C. Allen, The sodium- and potassium-activated adenosinetriphosphatase system, Meth. Pharmacol., 1: 361–388 (1971).Google Scholar
  9. 9.
    E. Amundsen, J. Puter, P. Friberger, M. Knos, M. Larsbraten, and G. Claeson, Methods for the determination of glandulary kallikrein by means of a chromogenic tripeptide substrate, Adv. Exp. Med. Biol., 120A: 83–95 (1979)Google Scholar
  10. 10.
    K. Yamada and E. Erdös, Isolation of two forms of kallikreins from rat kidney, Adv. Exp. Med. Biol., 156A: 387–391 (1983).PubMedGoogle Scholar
  11. 11.
    C. P. Vio, C. D. Figueroa, and I. Caorsi, Renal kallikrein: Cellular localization and subcellular distribution by ultrastructural immunocytochemistry, This Proceedings.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Knut-Jan Andersen
    • 1
  • Jarle Ofstad
    • 1
  1. 1.Medical Department A Haukeland SykehusUniversity of BergenBergenNorway

Personalised recommendations