Purification of Inactive Kallikrein from Rat Urine
An inactive kallikrein was purified from rat urine, and some of the properties of this enzyme were examined, in comparison with those of rat urinary kallikrein (RUK). The purified inactive kallikrein reacted with the antiserum against RUK and migrated slightly more slowly than RUK, on the Immunoelectrophoresis. The molecular weights of the inactive kallikrein and RUK were estimated to be 44,000 and 38,000 by gel filtration, respectively. These results indicate that the rat urinary inactive kallikrein is immunologically identical with RUK, but this inactive enzyme has biochemical properties different from those of RUK, with respect to molecular weight and electrophoretical mobility.
KeywordsKidney Slice Immunodiffusion Analysis Amino Acid Aspartic Acid Molecular Weight MOlecular Weight Specific Protein Activity Activity
Unable to display preview. Download preview PDF.
- 3.A. G. Scicli, O. A. Carretero, A. Hampton, P. Cortes, and N. Oza, Site of kininogenase secretion in the dog nephron, Am. J. Physiol. 230: 533–536.Google Scholar
- 4.J. Roblero, H. Croxatto, R. Garcia, J. Corthorn, and E. De Vito, Kallikrein activity in perfusates and urine of isolated rat kidneys, Am. J. Physiol. 231: 1383–1389.Google Scholar
- 5.J. J. Pisano, J. Corthorn, K. Yates, and J. V. Pierce, The kallikrein-kinin system in the kidney, Contr. Nephrol. 12: 116–125, (1978).Google Scholar
- 6.J. Corthorn, T. Imanari, H. Yoshida, T. Kaizu, J. V. Pierce, and J. J. Pisano, Isolation of prokallikrein from human urine, Adv. Exp. Med. Biol. 120: 575–579, (1979).Google Scholar
- 8.H. Nolly and M. C. Lama, Active and inactive kallikrein released by kidney slices from normotensive and hypertensive rats, Hypertension. 3 (Suppl): 35–38, (1981).Google Scholar
- 9.J. Corthorn, L. Bio, and J. Roblero, Release of activatable kallikrein by isolated rat kidneys, Hypertension. 3 (Suppl): 39–41, (1981).Google Scholar
- 11.B. H. Van Leeuwen, S. M. Crinblat, and C. I. Johnston, Release of active and inactive kallikrein from the isolated perfused rat kidney, Clin. Sci. 66: 207–215, (1984).Google Scholar
- 13.D. H. Spackman, W. H. Stein, and S. Moore, Automatic recording apparatus for use in the chromatography of amino acids, Anal. Chem. 30: 1190–1206, (1958).Google Scholar
- 14.O. Ouchterlony, Diffusion-in-gel methods for immunological analysis, in: Progress in allergy, vol. 5, ed. by P. Kallos and B. H. Waksman, Kargel, pp. 1–78, (1958).Google Scholar
- 20.H. Okamura, M. Takaoka, T. Iwamoto, and S. Morimoto, Renal inactive kallikrein as the possible origin of urinary inactive kallikrein in the rat, J. Pharm. Dyn. 8:in press, (1985).Google Scholar