Advertisement

Kinins IV pp 235-239 | Cite as

Receptor-Mediated Clearance of Tissue Kallireins by Rat Liver

  • Durval R. Borges
  • Maria Kouyoumdjian
  • Eline S. Prado
  • José Leal Prado
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)

Summary

The exsanguinated, isolated and perfused rat liver clears from the perfusate, at comparable rates, some native tissue kallikreins: human and horse urinary as well as hog pancreatic; the clearance rates were dependent on the initial enzyme concentration in the perfusing fluid. Contrarywise, rat urinary kallikrein was cleared at negligible rates. Neuraminidase pretreatment of these four kallikreins did not alter their clearance rates.

Horse urinary kallilrein binding to isolated prefixed hepatocytes was calcium-dependent and inhibited by asialofetuin (but not by fetuin) and some sugars; these characteristics are compatible with the interpretation that this native tissue kallikrein is recognized by the hepatocyte asialoglycoprotein-receptor. It was calculated that there are about 300, 000 receptor sites per cell either using perfusion experiments at 4°C or isolated hepatocytes.

Keywords

Sialic Acid Clearance Rate Sialic Acid Residue Perfusion Fluid Tissue Kallikrein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    D. R. Borges, M. E. Webster, J. A. Guimaraes, and J. L. Prado, Synthesis of prekallikrein and metabolism of plasma kallikrein by perfused rat liver, Biochem. Pharmacol. 30; 1065–1069 (1981).Google Scholar
  2. 2.
    D. R. Borges, A. H. Gordon, J. A. Guimaraes, and J. L. Prado, Recognition and catabolism of plasma kallikrein by perfused rat liver, in: “Recent Progress on Kinins,” H. Fritz, G. Dietze, F. Fiedler, and G. L. Haberland, eds., Birhäuser Verlag, Basel, Boston, Stuttgart (1982).Google Scholar
  3. 3.
    D. R. Borges, A. H. Gordon, J. A. Guimaräes, and J. L. Prado, Ratplasma kallikrein clearance by perfused rat liver, Brazilian J. Med. Biöl. Res. (In press, 1984).Google Scholar
  4. 4.
    D. R. Borges, C. Sampaio, P. De la Llosa, and J. L. Prado, The liver is the main organ to clear plasma and tissue kallikreins from rat plasma, in vivo, ( Accompanying paper, 1985 ).Google Scholar
  5. 5.
    G. Ashwell and J. Harford, Carbohydrate-specific receptors of the liver, Ann, Rev. Biochem. 51: 531–534 (1982).CrossRefGoogle Scholar
  6. 6.
    R. J. Stockert and A. G. Morell, Hepatic binding protein: the galactose-specific receptor of mammalian hepatocytes, Hematology 3: 750–757 (1983).Google Scholar
  7. 7.
    W. A. Dunn and A. L. Hubbard, Receptor-mediated endocytosis of epidermal growth factor by hepatocytes in the perfused rat liver: ligand and receptor dynamics, J. Cell Biol, 98: 2148–2159 (1984).PubMedCrossRefGoogle Scholar
  8. 8.
    S. Matsuura, H. Nakala, T. Sawamura, and Y. Tashiro, Distribution of an asialoglycoprotein receptor on rat hepatocyte cell surface, J. Cell Biol, 95: 864–875 (1982).PubMedCrossRefGoogle Scholar
  9. 9.
    H. Tunes, E. Silva, and M. Mares-Guia, Alpha- and beta-rat urinary kallikreins: chemical and physiocochemical properties, Brazilian J. Med, Biol. Res. 16: 193–202 (1983).Google Scholar
  10. 10.
    E. S. Prado, L. Juliano, M. Araujo-Viel, and M. A. Juliano, Characterization of kallikreins by model oligopeptides (In these proceedings, 1985).Google Scholar
  11. 11.
    E. S. Prado, C. A. M. Sampaio, M. S. Araüjo-Viel, and R. C. R. Stella, Characterization of horse urinary kallikrein, in: “Recent Progress on Kinins,” H. Fritz, G. Dietze, F. Fiedler, and G. L. Haberland, eds., Birhäuser Verlag, Basel, Boston, Stuttgart (1982).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Durval R. Borges
    • 1
  • Maria Kouyoumdjian
    • 2
  • Eline S. Prado
    • 2
  • José Leal Prado
    • 2
  1. 1.Department of MedicineEscola Paulista de MedicinaSão PauloBrazil
  2. 2.Department of BiochemistryEscola Paulista de MedicinaSão PauloBrazil

Personalised recommendations