Receptor-Mediated Clearance of Tissue Kallireins by Rat Liver
The exsanguinated, isolated and perfused rat liver clears from the perfusate, at comparable rates, some native tissue kallikreins: human and horse urinary as well as hog pancreatic; the clearance rates were dependent on the initial enzyme concentration in the perfusing fluid. Contrarywise, rat urinary kallikrein was cleared at negligible rates. Neuraminidase pretreatment of these four kallikreins did not alter their clearance rates.
Horse urinary kallilrein binding to isolated prefixed hepatocytes was calcium-dependent and inhibited by asialofetuin (but not by fetuin) and some sugars; these characteristics are compatible with the interpretation that this native tissue kallikrein is recognized by the hepatocyte asialoglycoprotein-receptor. It was calculated that there are about 300, 000 receptor sites per cell either using perfusion experiments at 4°C or isolated hepatocytes.
KeywordsSialic Acid Clearance Rate Sialic Acid Residue Perfusion Fluid Tissue Kallikrein
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