Kinins IV pp 219-228 | Cite as

Purification and Partial Characterization of Cat Pancreatic and Urinary Kallikreins — Comparison with Other Cat Tissue Kallikreins and Related Proteases

  • Hiroyuki Fujimori
  • Peter R. Levison
  • Melville Schachter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)


Kallikreins have been purified from cat pancreas and urine by methods similar to those described previously for cat colon and submandibular gland kallikreins.6 The pancreatic kallikrein (M.W. 41,200, pI 4.75) was similar to the urinary kallikrein (M.W. 34,300 pI 4.35–4.70) in pH optimum, substrate specificity and inhibition profile. Both enzymes were potent kininogenases and immunologically similar. These enzymes closely resembled the kallikreins from cat colon and submandibular glands. A trypsin (M.W. 18,800) was isolated from cat pancreas and shown to be distinct from the group of kallikreins in all parameters tested.. We attempted purification of cat renal kallikrein, but were unable to isolate any such enzyme. The major acidic esterase of cat kidney cortex (M.W. 59,000, pi 4.91) was purified and was distinct from both the cat tissue kallikreins and trypsin. The origin of cat urinary kallikrein remains unclear, but in the light of our findings, it may result from renal filtration of blood-borne tissue kallikreins rather than from intrarenal synthesis.


Submandibular Gland Tissue Kallikrein Bovine Trypsin Renal Filtration Urinary Kallikrein 
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  1. 1.
    O. A. Carretero, & A. G. Scicli, Renal kallikrein; its localization and possible role in renal function, Fed. Proc., 35: 194–198, (1976).PubMedGoogle Scholar
  2. 2.
    O. A. Carretero, & A. G. Scicli, The renal kallikrein-kinin system, Amer. J. Physiol., 238: F247–F255, (1980).Google Scholar
  3. 3.
    E. G. Erdos, & K. Yamada, Prekallikrein, kallikrein and renin in membrane fractions of rat kidney, Clin. Exp. Hypertens. A. Theor., 4: 2083–2096, (1982).CrossRefGoogle Scholar
  4. 4.
    F. Fiedler, Enzymology of glandular kallikreins, in: Bradykinin, kallidin and kallikrein ed. by E. G. Erdös, Handb. Exp. Pharmaco1., 25 Suppl., Springer-Verlag, Berlin, pp. 103–161, (1979).Google Scholar
  5. 5.
    F. Fiedler, & W. Gebhard, Isolation and characterization of native single-chain porcine pancreatic kallikrein, another possible precursor of urinary kallikrein, Hoppe-Seyler1s Z. Physiol. Chem., 361: 1661–1671, (1980).CrossRefGoogle Scholar
  6. 6.
    H. Fujimori, P. R. Levison, & M. Schachter, Purification and partial characterisation of cat colon and submandibular gland kallikreins, Adv. Exp. Med. Biol., (in press, 1985).Google Scholar
  7. 7.
    Y. Fukuoka, Y. Hojima, S. Miyaura, & C. Moriwaki, Purification of cat submaxillary kallikrein, J. Biochem., 85: 549–557, (1979).PubMedGoogle Scholar
  8. 8.
    R. Geiger, B. Clausnitzer, E. Fink, & H. Fritz, Isolation of an enzymatically active glandular kallikrein from human plasma by immunoaffinity chromatography, Hoppe-Seyler1s Z. Physiol. Chem., 361: 1795–1803, (1980).CrossRefGoogle Scholar
  9. 9.
    D. J. Holdstock, A. P. Mathias, & M. Schachter, A comparative study of kinin, kallidin and bradykinin, Brit. J. Pharmacol. Chemother., 12: 149–158, (1957).Google Scholar
  10. 10.
    W. J. Lawton, D. Proud, M. E. Freeh, J. V. Pierce, H. R. Keiser, & J. J. Pisano, Characterization and origin of immunoreactive glandular kallikrein in rat plasma, Biochem. Pharmacol., 30: 1731–1737, (1981).Google Scholar
  11. 11.
    H. S. Margolius, The kallikrein-kinin system and the kidney, Ann. Rev. Physiol., 46: 309–326, (1984).CrossRefGoogle Scholar
  12. 12.
    Y. Matsuda, K. Miyazaki, H. Moriya, & Y. Fujimoto, Human renalkallikrein: Purification and some properties, Chem. Pharm. Bull., 29: 2106–2108, (1981).PubMedCrossRefGoogle Scholar
  13. 13.
    C. Moriwaki, K. Miyazaki, Y. Matsuda, H. Moriya, Y. Fujimoto, & H. Ueki, Dog renal kallikrein: Purification and some properties, J. Biochem., 80: 1277–1285, (1976).PubMedGoogle Scholar
  14. 14.
    A. Nagamatsu, K. Abe, & S. Soeda, Purification and some properties of hog renal kallikrein, Chem. Pharm. Bull., 29: 1981–1985, (1981).PubMedCrossRefGoogle Scholar
  15. 15.
    L. Obika, Recent developments in urinary kallikrein research, Life Sci., 23: 764–775, (1978).CrossRefGoogle Scholar
  16. 16.
    G. S. Pinkus, O. Ole-Moi Yoi, K. F. Austen, & J. Spragg, Antigenic separation of a nonkinin-generating TAMe esterase from human urinary kallikrein and immunohistochemical comparison of their localization in the kidney, J. Histochem. Cytochem., 29: 38–44, (1981).Google Scholar
  17. 17.
    J. L. Prado, Proteolytic enzymes as kininogenases, Handb. Exp. Pharmacol., 25: 156 – 192, (1970).Google Scholar
  18. 18.
    S. F. Rabito, A, G. Scicli, V. Kher, & O, A, Carretero, Immunoreactive glandular kallikrein in rat plasma: a radioimmunoassay for its determination, Amer. J. Physiol., 242: H602–H610, (1982).PubMedGoogle Scholar
  19. 19.
    M. Schachter, Kallikreins (kininogenases) - a group of serine proteases with bioregulatory actions, Pharmacol. Rev., 31: 1–17, (1980).Google Scholar
  20. 20.
    M. Schachter, M. W. Peret, C. Moriwaki, & J. A. A. Rodrigues, Localization of kallikrein in submandibular gland of cat, guinea pig, dog and man by the immunoperoxidase method, J. Histochem. Cytochem., 28: 1295–1300, (1980).PubMedCrossRefGoogle Scholar
  21. 21.
    D. D. Schroeder, & E. Shaw, Chromatography of trypsin and its derivatives. Characterization of a new active form of bovine trypsin, J. Biol. Chem., 243: 2943–2949, (1968).PubMedGoogle Scholar
  22. 22.
    K. Shimamoto, R. K. Mayfield, H. S. Margolius, J. Chao, W. Stroud, & A. P. Kaplan, Immunoreactive tissue kallikrein in human serum, J. Lab. Clin. Med., 103: 731–738, (1984).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Hiroyuki Fujimori
    • 1
  • Peter R. Levison
    • 1
  • Melville Schachter
    • 2
  1. 1.Alberta Heritage Foundation for Medical Research FellowsEdmonton AlbertaCanada
  2. 2.Department of PhysiologyUniversity of AlbertaEdmontonCanada

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