Kinins IV pp 19-26 | Cite as

Intracellular and Intercellular Distributions of Acid Kininogenases in Spleen

  • Masako Watanabe
  • Keiko Yamafuji
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)


Intracellular distributions of acid kininogenases were investigated following the subcellular fractionation of bovine and rat spleens. Acid kininogenases as well as other acid hydrolases were observed to be distributed in several fractons. Relatively high kinin forming activity was found in two fractions which were considered to contain lysosomes from reticulum cells and from lymphocytes respectively. The latter fraction had shown the higher sensitivity to thiol compound for its activation. These findings led us to the separation of the free cells existing in spleen each other. We adopted the techniques such as Ficoll-Isopaque centrifugation, plastic dish treatment and iron carbonyl treatment for this purpose. Lymphocyte from rat thymus was also examined and proved to have acid kininogenase activity as well.


Subcellular Fractionation Mitochondrial Fraction Thiol Compound Acid Hydrolase Iron Carbonyl Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    K. Yamafuji and M. Takeishi, Substrate specificities of acid kininogenases, Adv. Exp. Med. Biol., 120A: 335 (1979).Google Scholar
  2. 2.
    Yamafuji, K. and M. Watanabe, Studies on the kinin formation by bovinespleen acid kininogenases, Adv. Exp. Med. Biol., 156A: 409 (1983).PubMedGoogle Scholar
  3. 3.
    H. Ragab, C. Beck, C. Dillard, and A. L. Tappel, Preparation of ratliver lysosomes, Biochem. Biophys. Acta., 148: 501 (1967).CrossRefGoogle Scholar
  4. 4.
    W. E. Bowers and C. de Duve, Lysosomes in lymphoid tissue II. Intracellular distribution of acid hydrolases, J. Cell. Biol., 32: 339 (1967).PubMedCrossRefGoogle Scholar
  5. 5.
    W. E. Bowers and C. de Duve, Lysosomes in lymphoid tissue III. Influence of various treatments of the animals on the distributionof acid hydrolases, J. Cell. Biol., 32: 349 (1967).PubMedCrossRefGoogle Scholar
  6. 6.
    L. M. Greenbaum and T. Hosoda, Studies on the isolation of bradykininogen, Biochem. Pharmacol., 12: 325 (1963).Google Scholar
  7. 7.
    S. Ohishi, Y. Uchida, A. Ueno, and M. Katori, Bromelain, athiol protease from pineapple stem, depleltion high molecular weight kininogen by activation of hageman factor (Factor XII), Thrombosis Research, 14: 665 (1979).CrossRefGoogle Scholar
  8. 8.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, Proteinmeasurement with Folin phenol reagent, J. Biol. Chem., 193: 263 (1951).Google Scholar
  9. 9.
    H. Harada, T. Kasahara, Y. Ito, and T. Kawai, Laboratory techniques for isolation, identification and removal of monocyte, Diagnostic Pathology (Rinsyo Byori in Japanese), Supplement: 87 (1981).Google Scholar
  10. 10.
    I. Ishikawa and G. Gimasoni, Isolation of Cathepsin D from human lymphocytes, Biochem. Biophys. Acta, 480: 228 (1977).CrossRefGoogle Scholar
  11. 11.
    T. Shiroishi, Preparation of lymph node. Protein, nucleic acid and enzyme, Supplement 24: Illustrated manual for animal experiment: 43, in Japanese (1981).Google Scholar
  12. 12.
    Y. Kagawa, Salt solutions, Biochemistry Data Book II: 924, Kagakudozin, in Japanese (1981).Google Scholar
  13. 13.
    Y. Orii, Purification and properties of bovine heart cytochrome oxydase. Protein, nucleic acid and enzyme, Supplement: Experiments in biological membrane II: 228, in Japanese (1974).Google Scholar
  14. 14.
    H. Fujiwara, Effects of chemical mediators (histamine, bradykinin, serotonin and acetylcholine) on lymphocyte activation by antigen or mitogen, Allergy, 31–1:29, in Japanese (1982).Google Scholar
  15. 15.
    Okabe, M. Hida, K. Takada, Y. Noose, M. Yamamoto, and Y. Kimura, Agents and action, Supplement 9: 315 (1982).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Masako Watanabe
    • 1
  • Keiko Yamafuji
    • 1
  1. 1.Department of Food and NutritionNakamura Gakuen CollegeFukuokaJapan

Personalised recommendations