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Kinins IV pp 19-26 | Cite as

Intracellular and Intercellular Distributions of Acid Kininogenases in Spleen

  • Masako Watanabe
  • Keiko Yamafuji
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)

Summary

Intracellular distributions of acid kininogenases were investigated following the subcellular fractionation of bovine and rat spleens. Acid kininogenases as well as other acid hydrolases were observed to be distributed in several fractons. Relatively high kinin forming activity was found in two fractions which were considered to contain lysosomes from reticulum cells and from lymphocytes respectively. The latter fraction had shown the higher sensitivity to thiol compound for its activation. These findings led us to the separation of the free cells existing in spleen each other. We adopted the techniques such as Ficoll-Isopaque centrifugation, plastic dish treatment and iron carbonyl treatment for this purpose. Lymphocyte from rat thymus was also examined and proved to have acid kininogenase activity as well.

Keywords

Subcellular Fractionation Mitochondrial Fraction Thiol Compound Acid Hydrolase Iron Carbonyl Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Masako Watanabe
    • 1
  • Keiko Yamafuji
    • 1
  1. 1.Department of Food and NutritionNakamura Gakuen CollegeFukuokaJapan

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