Kininogen by the SRI Method in Human Serum During an Acute Phase Inflammatory Reaction
The concentration of several serum proteins changes during the acute phase in inflammatory states.1 Kininogen belongs to this group of acute phase reactants as shown in our earlier studies and also with plasma from patients with rheumatoid arthritis.2 In early studies plasma kininogen was measured exclusively by estimation of the bradykinin equivalent by bio- assay on the isolated rat uterus or guinea pig ileum. 3 More recently a bradykinin enzyme immunoassay4, radioimmunoassay5 and rocket Immunoelectrophoresis6 were reported for the determination of HMr kininogen in plasma. A method designated to determination of the total plasma and serum kininogen by single radial immunodiffusion (SRI) was recently presented by us in detail.7 The SRI method was compared with and correlated to kininogen determined by the bradykinin equivalent and gives the corresponding normal values in human plasma and serum. This presentation deals with its application in a study of kininogen investigated in a serum material collected from patients during periods of treatment with immunotherapy in renal cell carcinoma.8–12 An infiammatory reaction provoked during the treatment was shown by the increase of a1-acid glycoprotein and some other acute phase reactants.
KeywordsRenal Cell Carcinoma Patient Seron Plasma Kallikrein High Molecular Weight Kininogen Active Specific Immunotherapy
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