Kinins IV pp 155-160 | Cite as

Studies on the Antigenic Determinants of Human Low Molecular Weight Kininogen

  • Tytti Kärkkäinen
  • Ann-Christine Syvänen
  • Ulla Hamberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)


The antigenic reactivity of proteins is located in restricted parts of the molecule forming the antigenic determinants that react with the binding sites of the antibody molecule.1 Low molecular weight (LMr) and high molecular weight (HMr) kininogens are immunologically related molecules with common antigenic determinants in the heavy chain of both proteins. 2–4 We have further characterized the nature of these determinants using a kinin-free LMr kininogen isolated from Cohns plasma fraction IV (He antigen). 5,6 shown recently7 the determinant structure of kininogen reside in the polypeptide backbone of the molecule and is not influenced by the partial removal of carbohydrates. This antigen has a heterogeneous light chain which can be isolated after reduction and carboxymethylation. An antiserum produced against the native HC antigen recognizes all native forms of kininogen in human plasma.


High Performance Liquid Chromatography Heavy Chain Antigenic Determinant Antigenic Structure Antigenic Response 
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  1. 1.
    M. Z. Atassi, Precise determination of the entire antigenic structure of lysozyme: Molecular features of protein antigenic structures and potential of surface-stimulation synthesis - a powerful new concept for protein binding sites, Immunochemistry, 15: 909–926, (1975).CrossRefGoogle Scholar
  2. 2.
    H. Z. Habal, B. J. Underdown, and H. Z. Movat, Further characterization of human plasma kininogen, Biochem. Pharmac., 24: 1241–1243, (1975).CrossRefGoogle Scholar
  3. 3.
    D. M. Kerbiriou, B. N. Bouma, and J. H. Griffin, Immunochemical studies of human high molecular weight kininogen and of its complexes with plasma prekallikrein and kallikrein, J. Biol. Chem., 255: 3952–3958, (1980).PubMedGoogle Scholar
  4. 4.
    A.-C. Syvanen, U. Turpeinen, S. Siimesmaa, and U. Hamberg, A radio immunoassay for the detection of molecular forms of human plasma kininogen, FEBS Lett., 129: 241–245, (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    A.-C. Syvanen, T. Karkkainen, and U. Hamberg, Conformation and sequence dependent antigenic determinants in human low molecular weight kininogen, Mol. Immunol., 20: 669–678, (1983).Google Scholar
  6. 6.
    T. Kärkkäinen, A.-C., Syvanen, U. Turpeinen, and U. Hamberg, Isolation and immunologic properties of a heterogeneous antigen with the characteristics of the heavy chain of human low molecular weight kininogen, Mol. Immunol., 19: 179–189, (1982).Google Scholar
  7. 7.
    U. Turpeinen, Studies on the antigenicity and carbohydrates of human low molecular weight kininogen, Mol. Immunol., 20: 1411–1418, (1983).Google Scholar
  8. 8.
    T. Karkkainen, Immunopurification of human plasma kininogens, Mol. Immunol., in press.Google Scholar
  9. 9.
    J.-P. Bouvet, J. Pillot, and P, Liacopoulos, Human myeloma light chains with increased molecular weight: high frequency among A chains, Mol. Immunol., 20: 397–407, (1983).Google Scholar
  10. 10.
    F. C. Greenwood, W. M. Hunter, and J. S. Glover, The preparation of 131I-labelled human growth hormone of high specific radioactivity, Biochem. J., 89: 114–123, (1963).PubMedGoogle Scholar
  11. 11.
    B. Desbuquois and G. D. Aurbach, Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunor- assays, J. Clin. Endocrinol., 33: 732–738, (1971).CrossRefGoogle Scholar
  12. 12.
    U. K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227: 680–685, (1970).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Tytti Kärkkäinen
    • 1
  • Ann-Christine Syvänen
    • 1
  • Ulla Hamberg
  1. 1.Department of BiochemistryUniversity of HelsinkiHelsinki 17Finland

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