Kininogenase from Rat Vascular Tissue
A kininogenase resembling glandular kallikrein ws partially purified from vascular tissue and characterized. Saline perfused rat tail arteries and veins were homogenized in 0.25 M sucrose containing 10 mM Tris-HC1 (pH 7.4). The homogenate was centrifuged at 105,000 x g for 60 min and a vascular kininogenase was purified from the supernatant by chromatofocusing, affinity chromatography on immobilized antibodies against rat urinary kallikrein, and gel filtration on Sephadex G-100. The inhibitory effects of antibodies against rat urinary kallikrein were tested using equivalent kininforming concentrations of rat urinary kallikrein and vascular kininogenase. Kininogenase activities of both enzymes were similarly inhibited by urinary kallikrein antibodies. Aprotinin (1,000 KIU) completely inhibited vascular kininogenase activity while soybean trypsin inhibitor (100 µg) did not modify its kinin-forming activity. Vascular kininogenase and rat urinary kallikrein had the same elution volume when chromatographed on a Sephadex G-100 column and had similar mobilities in 10% polyacrylamide gel electrophoresis. Kinins released by vascular kininogenase were identified as bradykinin by reverse-phase high performance liquid chromatography. Rat vascular kininogenase appears to be similar to glandular kallikrein. Kinins released locally by vascular kininogenase may contribute to the regulation of vascular tone.
KeywordsHigh Performance Liquid Chromatography Elution Volume Soybean Trypsin Inhibitor Plasma Kallikrein Urinary Kallikrein
Unable to display preview. Download preview PDF.
- 1.M. E. Webster, Kallikrein in glandular tissue, in: “Handbook of Experi mental Pharmacology, Bradykinin, Kallidin and Kallikrein,” E. G. Erdos, ed., Springer, Berlin, Heidelberg, New York (1970).Google Scholar
- 2.S. F. Rabito, A. G. Scicli, and O. A. Carretero, Immunoreactive glandular kallikrein in plasma, in: “Enzymatic Release of Vasoactive Peptides,” F. Gross and H. G. Vogel, eds., Raven Press, New York (1980).Google Scholar
- 4.H. Nolly and M. C. Lama, Vascular kallikrein: A kallikrein-like enzyme present in vascular tissue of the rat, Clin. Sci., 63: 249s–251s (1968).Google Scholar
- 6.O. A. Carretero, N. B. Oza, A. Piwonska, T. Ocholik, and A. G. Scicli, Measurement of urinary kallikrein activity by kinin radioimmunoassay, Biochem. Pharmacol., 25: 2265–2270 (1976).Google Scholar