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Kinins IV pp 11-17 | Cite as

Kininogenase from Rat Vascular Tissue

  • Hector Nolly
  • A. Guillermo Scicli
  • Gloria Scicli
  • M. Cristina Lama
  • Ana M. Guercio
  • Oscar A. Carretero
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)

Summary

A kininogenase resembling glandular kallikrein ws partially purified from vascular tissue and characterized. Saline perfused rat tail arteries and veins were homogenized in 0.25 M sucrose containing 10 mM Tris-HC1 (pH 7.4). The homogenate was centrifuged at 105,000 x g for 60 min and a vascular kininogenase was purified from the supernatant by chromatofocusing, affinity chromatography on immobilized antibodies against rat urinary kallikrein, and gel filtration on Sephadex G-100. The inhibitory effects of antibodies against rat urinary kallikrein were tested using equivalent kininforming concentrations of rat urinary kallikrein and vascular kininogenase. Kininogenase activities of both enzymes were similarly inhibited by urinary kallikrein antibodies. Aprotinin (1,000 KIU) completely inhibited vascular kininogenase activity while soybean trypsin inhibitor (100 µg) did not modify its kinin-forming activity. Vascular kininogenase and rat urinary kallikrein had the same elution volume when chromatographed on a Sephadex G-100 column and had similar mobilities in 10% polyacrylamide gel electrophoresis. Kinins released by vascular kininogenase were identified as bradykinin by reverse-phase high performance liquid chromatography. Rat vascular kininogenase appears to be similar to glandular kallikrein. Kinins released locally by vascular kininogenase may contribute to the regulation of vascular tone.

Keywords

High Performance Liquid Chromatography Elution Volume Soybean Trypsin Inhibitor Plasma Kallikrein Urinary Kallikrein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Hector Nolly
    • 1
  • A. Guillermo Scicli
    • 2
  • Gloria Scicli
    • 2
  • M. Cristina Lama
    • 1
  • Ana M. Guercio
    • 1
  • Oscar A. Carretero
    • 2
  1. 1.Department of PathologySchool of Medicine Universidad Nacional de CuyoMendozaArgentina
  2. 2.Hypertension Research DivisionHenry Ford HospitalDetroitUSA

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