Kinins IV pp 113-118 | Cite as

Independent Consumption of High and Low Molecular Weight Kininogens in Vivo

  • Yasuhiro Uchida
  • Makoto Katori
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)


The levels of high molecular weight (HMW) kininogen and pre-kallikrein in rat plasma were markedly reduced after single injection of bromelian (10 mg/kg, i. v.) and gradually recovered over a 72 hour period. The level of low molecular weight (LMW) kininogen, however, was not changed during this period.

Rat pleurisy was induced by intrapleural injection of λ-carrageenin. The levels of HMW kininogen and prekallikrein, but not of LMW kininogen, in the exudate were markedly decreased, when compared with those in plasma of the same animals.

After pretreatment with disulfiram, oral administration of ethanol (2 g/kg) or intravenous injection of acetaldehyde (10 mg/kg) to rats caused significant effect on the plasma HMW kininogen and prekallikrein levels.

These results suggest that HMW and LMW kininogens may be consumed separately in vivo and play different roles.


Tissue Kallikrein Plasma Kallikrein High Molecular Weight Kininogen Pleural Exudate Intrapleural Injection 
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  1. 1.
    E. Haberman, Kininogens, in: Handbook of Experimental Pharmacology, XXV, ed. by E. G. Erdoes, Springer-Verlag, Berlin, pp. 250–288, (1970).Google Scholar
  2. 2.
    K. D. Wuepper, Prekallikrein deficiency in man, J. Exp. Med., 138: 1345–1355, (1973).PubMedCrossRefGoogle Scholar
  3. 3.
    H. Saito, O. D. Ratnoff, R. Waldmann, and J. P. Abraham, Fitzgerald trait: Deficiency of a hitherto unrecognized agent, Fitzgerald factor, participating in surface-mediated reactions of clotting, fibrinolysis, generation of kinins, and the property of diluted plasma enhancing vascular permeability (PF/DIL), J. Clin. Invest., 55: 1082–1089, (1975).PubMedCrossRefGoogle Scholar
  4. 4.
    R. W. Colan, A. Bagdasarian, R. C. Talamo, C. F. Scott, A. M. Seavey, J. A. Guimaraes, J. V. Pierce, and A. P. Kaplan, Williams trait: Human kininogen deficiency with diminished levels of plasminogen proactivator and prekallikrein associated with abnormalities of the Hageman factor-dependent pathways, J. Clin. Invest., 56: 1650–1662, (1975).CrossRefGoogle Scholar
  5. 5.
    S. Oh-ishi, A. Ueno, Y. Uchida, M. Katori, H. Hayashi, H. Koya, K. Kitajima, and I. Kimura, Abnormalities in the contact activation through factor XII in Fujiwara trait: A deficiency in both high and low molecular weight kininogens with low level of prekallikrein, Tohoku J. Exp. Med., 133: 67–80, (1981).Google Scholar
  6. 6.
    S. Oh-ishi and M. Katori, Fluorometric assay for plasma prekallikrein using peptidylmethylcoumarinylamide as a substrate, Thrombosis Res., 14: 551–559, (1979).CrossRefGoogle Scholar
  7. 7.
    Y. Uchida and M. Katori, Differential assay method for high molecular weight and low molecular weight kininogens, Thrombosis Res., 15: 127–134, (1979).CrossRefGoogle Scholar
  8. 8.
    H. Okamoto and L. M. Greenbaum, Kininogen substrates for trypsin and cathepsin D in human, rabbit and rat plasmas, Life Sci., 32: 2007–2013, (1983).PubMedCrossRefGoogle Scholar
  9. 9.
    S. Oh-ishi, Y. Uchida, A. Ueno, and M. Katori, Bromelain, a thiol- protease from pineapple stem, depletes high molecular weight kininogen by activation of Hageman factor (factor XII), Thrombosis Res., 14: 665–672, (1979).CrossRefGoogle Scholar
  10. 10.
    Y. Uchida, K. Tanaka, Y. Harada, A. Ueno, and M. Katori, Activation of plasma kallikrein-kinin system and its significant role in pleural fluid accumulation of rat carrageenin-induced pleurisy, Inflammation, 7: 121–131, (1983).PubMedCrossRefGoogle Scholar
  11. 11.
    K. Hatake, Behavior of the kallikrein-kinin system on alcohol ingestion, Jpn. J. Alcohol & Drug Dependence, 19: 32–62, (1984) (in Japanese).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Yasuhiro Uchida
    • 1
  • Makoto Katori
    • 1
  1. 1.Department of PharmacologyKitasato University School of MedicineSagamihara, Kanagawa 228Japan

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