Kinins IV pp 77-84 | Cite as

Characterization of Kininogen Deficiency of Brown Norway Rat Mutant Katholiek Strain

  • Sachiko Oh-ishi
  • Izumi Hayashi
  • Takekazu Ino
  • Hisao Kato
  • Sadaaki Iwanaga
  • Takeshi Nakano
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)


A deficiency in the plasma kallikrein-kinin system of Brown Norway rat mutant Katholiek strain (B/N-Ka), first reported by Damas et al. was further characterized. The prolonged activated partial thromboplastin time (APTT) of B/N-Ka rat plasma was corrected by an addition of rat HMW- kininogen, indicating B/N-Ka rat is deficient in HMW kininogen.

The study of kinin-release of plasmas of the three strains of rat (B/N-ka, B/N Ki and SD) by several kininogenases expressed that B/N-Ka rat is deficient in LMW kininogen, in addition to HMW kininogen deficiency.

The plasmas of the three strains of rat were gel-filtered through Sephacryl S-200 gel and profiles of kinin-release of the fractions were examined by several kininogenases. The result demonstrated that normal rat plasma contains three kinds of kininogen (HMW and LMW kininogens, and T-kininogen), and B/N-Ka plasma contains only T-kininogen.

B/N-Ka rat plasma demonstrated T-kininogen antigen but no HMW kininogen by the study of immunodiffusion using their antisera raised in rabbits.


Activate Partial Thromboplastin Time Lower Molecular Weight Fraction Plasma Kallikrein High Molecular Weight Kininogen Urinary Kallikrein 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Sachiko Oh-ishi
    • 1
  • Izumi Hayashi
    • 1
  • Takekazu Ino
    • 1
  • Hisao Kato
    • 2
  • Sadaaki Iwanaga
    • 2
  • Takeshi Nakano
    • 3
  1. 1.Department of Pharmacology, School of Pharmaceutical SciencesKitasato UniversityTokyo 108Japan
  2. 2.Department of Biology, Faculty of ScienceKyushu UniversityFukuoka 812Japan
  3. 3.Department of Experimental Animal Sciences, School of MedicineKitasato UniversitySagamihara 228Japan

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