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Kinins IV pp 69-75 | Cite as

Isolation and Properties of Two Rat Plasma T-Kininogens

  • Hiroshi Okamoto
  • Lowell M. Greenbaum
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)

Abstract

Two species of T-kininogen which release T-kinin (Ile-Ser-bradykinin) have been purified from plasma of rats treated with Freund’s complete adjuvant. The molecular weight was estimated to be 69,000 for either T-kininogen I and II by SDS-polyacrylamide gel electrophoresis. Trypsin released one mole of T-kinin from one mole of either T-kininogen, but glandular kallikrein, including rat urinary and rat submandibular gland kallikreins and human urinary kallikrein, did not release any kinin from T-kininogens. Cathepsin D, which was purified from rat liver, released T-kinin from T-kininogens at pH 4.0. These results indicate that rat plasma contains two types of T-kininogen which differ from high molecular weight and low molecular weight kininogens.

Keywords

Adjuvant Arthritis Dialysis Buffer Urinary Kallikrein Glandular Kallikrein Kinin Liberation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Hiroshi Okamoto
    • 1
  • Lowell M. Greenbaum
    • 1
  1. 1.Department of Pharmacology and ToxicologyMedical College of GeorgiaAugustaUSA

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