Kinins IV pp 69-75 | Cite as

Isolation and Properties of Two Rat Plasma T-Kininogens

  • Hiroshi Okamoto
  • Lowell M. Greenbaum
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 198A)


Two species of T-kininogen which release T-kinin (Ile-Ser-bradykinin) have been purified from plasma of rats treated with Freund’s complete adjuvant. The molecular weight was estimated to be 69,000 for either T-kininogen I and II by SDS-polyacrylamide gel electrophoresis. Trypsin released one mole of T-kinin from one mole of either T-kininogen, but glandular kallikrein, including rat urinary and rat submandibular gland kallikreins and human urinary kallikrein, did not release any kinin from T-kininogens. Cathepsin D, which was purified from rat liver, released T-kinin from T-kininogens at pH 4.0. These results indicate that rat plasma contains two types of T-kininogen which differ from high molecular weight and low molecular weight kininogens.


Adjuvant Arthritis Dialysis Buffer Urinary Kallikrein Glandular Kallikrein Kinin Liberation 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Hiroshi Okamoto
    • 1
  • Lowell M. Greenbaum
    • 1
  1. 1.Department of Pharmacology and ToxicologyMedical College of GeorgiaAugustaUSA

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