Purification of Human Gamete Antigens Relevant to Antibody Production: Application of Monoclonal Antibodies
Since Shivers and Dunbar1 reported the presence of antibodies cross reactive to P-ZP in some sterile women, Mori et al.2 and others3, 4, 5, 6 also reported similar results even after absorption with porcine red blood cells (rbc). However, several contradictory results were obtained by Sacco et al.7, Dakhno et al.8, Dietle et al.9 and others10,11. Therefore, we studied to confirm these questions.
KeywordsZona Pellucida Human Spermatozoon Antigenic Epitope Immunoaffinity Chromatography Human Seminal Plasma
Unable to display preview. Download preview PDF.
- 19.Dunbar, B. S., Liu, C., Sammons, D. W. Identification of the three major proteins of porcine and rabbit zonae pellucidae by high resolution two dimentional gel electrophoresis: Comparison with serum, follicular fluid, and ovarian cell proteins. Biol. Reprod., 24: 1111–1124, 1981.PubMedGoogle Scholar
- 22.Koyama, K., Hasegawa, A., Tsuji, Y., Isojima, S. Production and characterization of monoclonal antibodies to cross-reactive antigens of human and porcine zonae pellucidae. J. Reprod. Immunol. (In press), 1985.Google Scholar
- 24.Isojima, S., Koyama, K., Tsuchiya, K. The effect on fertility in women of circulating antibodies against human spermatozoa. J. Reprod. Fertil., Suppl., 21: 125–150, 1974.Google Scholar