Antigenic Profile and Impact of Immunization with Zona Pellucida Antigens in Primates
Within the past 10–12 years numerous studies have been published which describe the investigation of zona pellucida antigens for immunocontraceptive purposes (for reviews see Sacco, 1981; Dunbar, 1983). The earliest of these studies primarily used antigens associated with rodent zonae as immunogens and involved the use of antisera developed against total zona proteins. These early in vitro experiments and passive immunization studies clearly demonstrated the contraceptive potential of antibodies produced against the zona pellucida. When the immunologic similarity of antigens present in porcine and human zona was demonstrated (Sacco, 1977), the porcine zona became a prime candidate for consideration as a target antigen for the development of a human contraceptive vaccine. The availability of large numbers of porcine ovaries as a source of zonae made this species particularly attractive for immunocontraceptive studies.
KeywordsActive Immunization Zona Pellucida Immunization Study Ovarian Follicular Development Normal Ovarian Function
Unable to display preview. Download preview PDF.
- Dietl, J., Freye, J. and Mettler, L. (1982). Fertility inhibition using low-dose immunization with porcine zona pellucida. A.J.R.I. 2: 153–156.Google Scholar
- Dunbar, B.S. (1983). Antibodies to zona pellucida antigens and their role in fertility. In: Immunology of Reproduction, T. Wegmann, T. Gill, C. Cumming and E. Nisbet-Brown, eds., Oxford University Press, pgs. 506–534.Google Scholar
- Gulyas, B.J., Yuan, L.C., Gwatkin, R.B.L., and Schmell, E.D. (1983a). Response of monkeys to porcine zona pellucida as detected by a solidphase radioimmunoassay. J. Med. Primatol. 12: 332–342.Google Scholar
- Isojima, S., Koyama, K., and Hasegawa, A. (1981). Production of monoclonal antibodies to zona pellucida from porcine oocytes. Acta. Obst. Gynaec. Jpn. 33: 1995–1996.Google Scholar
- Mahi-Brown, C.A., Yanagimachi, R., Hoffmann, J.D., and Huang, T.T.F. (1985). Fertility control in the bitch by active immunization with porcine zonae pellucidae: use of different adjuvants and patterns of estradiol and progesterone levels in estrous cycles. Biol. Reprod. 32: 761–772.PubMedCrossRefGoogle Scholar
- Sacco, A.G. (1981). Immunocontraception: consideration of the zona pellucida as a target antigen. In: Obstetrics and Gynecology Annual, R.M. Wynn, ed., Vol. 10: 1–26.Google Scholar
- Shivers, C.A., Sieg, P.M., and Kitchen, H. (1981). Pregnancy prevention in the dog: Potential for an immunological approach. J. Amer. An. Hosp. Assoc. 17: 823–828.Google Scholar
- Skinner, S.M. and Dunbar, B.S. (1984) Comparison of immunization using a purified zona pellucida (ZP) protein or total ZP protein on ovarian follicular development. Biol. Reprod. 30 (Supplement 1): 73.Google Scholar
- Yurewicz, E.C., Sacco, A.G. and Subramanian, M.G. (1984a). Purification and characterization of glycoprotein antigens of the porcine zona pellucida. Fed. Proc. 43: 2039.Google Scholar
- Yurewicz, E.C., Sacco, A.G. and Subramanian, M.G. (1984b). Structure of the porcine zona pellucida: isolation and properties of the major glycoprotein family. J. Cell Biol. 99: 73a.Google Scholar