Advertisement

Toward the Development of an Anti-Sperm Contraceptive Vaccine

  • Rajesh K. Naz
Part of the Reproductive Biology book series (RBIO)

Abstract

Development of a vaccine based on sperm antigens represents a promising approach to contraception. The feasibility of this approach is based upon two evidences. The first type of evidence comes from the studies of involuntary immunoinfertility in humans. Presence of antisperm antibodies have been reported to be one of the major causes of immunoinfertility in both men and women.1 Second type of evidence is provided by the experimental immunization studies. Immunization of male and female animals of various species with extracts of sperm or mature testis results in a significant reduction in fertility by causing either fertilization failure or preimplantation embryo mortality or both. 2–5 The whole sperm cannot be used as an immunogen for the development of an antisperm vaccine due to the presence of numerous antigens on germ cells which could be shared with other somatic tissues. The utility of an antigen as an immunocontraceptive vaccine is contingent upon its tissue-specificity, its homogeneity, strong immunogenicity and its involvement in fertilization and fertility.

Keywords

Zona Pellucida Female Rabbit Antisperm Antibody Sperm Plasma Membrane Strong Immunogenicity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A.C. Menge, Clinical immunologic infertility: Diagnostic measures, incidence of antisperm antibodies, fertility mechanisms, in: “Immunological Aspects of Infertility and Fertility Regulation,” D.S. Dhindsa and G.F.B. Schumacher, eds., Elsevier-North Holland, New York (1980).Google Scholar
  2. 2.
    S. Katsh, Infertility in female guinea pigs induced by injection of homologous sperm, Am. J. Obstet. Gynecol. 78:276 (1959).PubMedGoogle Scholar
  3. 3.
    R.G. Edwards, Immunological control of fertility in male mice, Nature (London) 203, 50 (1964).PubMedCrossRefGoogle Scholar
  4. 4.
    C.A. Kiddy, and R.M. Rollins, Infertility in female guinea pigs injected with testis, Biol. Reprod. 8, 545 (1973).PubMedGoogle Scholar
  5. 5.
    A.C. Menge, Immune reaction and infertility, J. Reprod. Fertil. Suppl. 10, 171 (1970).Google Scholar
  6. 6.
    F.N. Syner, B.S. Kuros, and K.S. Moghissi, Active immunization of female rabbits with purified rabbit acrosin and effect on fertility, Fertil Steril, 32, 468 (1979).PubMedGoogle Scholar
  7. 7.
    D.B. Morton, and P.A. McAnulty, The effect on fertility of immunizing female sheep with ram sperm acrosin and hyaluronidase, J. Reprod. Immunol. 1, 61 (1979).PubMedCrossRefGoogle Scholar
  8. 8.
    M.G. O’Rand, Inhibition of fertility and sperm-zona binding of antiserum to the rabbit sperm membrane autoantigen RSA-1, Biol. Reprod. 25, 621 (1981).PubMedCrossRefGoogle Scholar
  9. 9.
    E. Goldberg, Effects of immunization with LDH-X on fertility, in: “Immunologic Approaches to Fertility Control,” E. Diczfalusy, ed., Karolinska Institute, Stockholm (1974).Google Scholar
  10. 10.
    E. Goldberg, T.E. Wheat, J.E. Powell, and V.C. Stevens, Reduction of fertility in female baboons immunized with lactate dehydrogenase-C-4, Fertil. Steril. 35, 214 (1981).PubMedGoogle Scholar
  11. 11.
    G. Kohler, and C. Milstein, Derivation of specific antibodyproducing tissue culture and tumor lines by cell fusion, Eur. J. Immunol. 6, 511 (1976).PubMedCrossRefGoogle Scholar
  12. 12.
    R.K. Naz, J.M. Saxe, and A.C. Menge, Inhibition of fertility in rabbits by monoclonal antibodies against sperm, Biol. Reprod. 28, 249 (1983).PubMedCrossRefGoogle Scholar
  13. 13.
    J. Friberg, A simple and sensitive micro method for demonstration of sperm — agglutinating activity in serum from infertile men and women, Acta Obstet. Gynecol. Scand. 74, 21 (1974).CrossRefGoogle Scholar
  14. 14.
    S. Isojima, T.S. Li, and Y. Ashitaka, Immunological analysis of sperm — immobilizing factor found in sera of women with unexplained infertility, Am. J. Obstet. Gynecol. 101, 677 (1968).Google Scholar
  15. 15.
    R.K. Naz, C.R. Bradford, and A.C. Menge, Isoantigenicity of rabbit sperm, testis and their extracts as demonstrated by Western blot enzyme immunobinding procedure, Arch.Androl. 13, 279 (1985).CrossRefGoogle Scholar
  16. 16.
    R.K. Naz, B.B. Rosenblum, and A.C. Menge, Characterization of a membrane antigen from rabbit testis and sperm isolated by using monoclonal antibodies and effect of its antiserum on fertility, Proc. Natl. Acad. Sci., U.S.A. 81, 857 (1984).PubMedCrossRefGoogle Scholar
  17. 17.
    R.K. Naz, R.J. Poffenberger, and A.C. Menge, Reduction of fertility in female rabbits and mice actively immunized with a germ-cell antigen (GA-1) from the rabbit, Biol. Reprod. Submitted.Google Scholar
  18. 18.
    R.K. Naz, N.J. Alexander, M. Isahakia, and M.S. Hamilton, Monoclonal antibody against a human germ cell membrane glycoprotein that inhibits fertilization, Science 225, 342 (1984).PubMedCrossRefGoogle Scholar
  19. 19.
    R.K. Naz, T.M. Phillips, and B.B. Rosenblum, Characterization of the fertilization antigen (FA-1) isolated from murine testis plasma membrane for the development of an immunocontraceptive vaccine, Proc. Natl. Acad. Sci. U.S.A. Submitted.Google Scholar
  20. 20.
    R.K. Naz, B.A. Littman, and R.J. Stillman, Successful human pregnancy following in vitro fertilization using frozen semen, J. IVF, in press.Google Scholar
  21. 21.
    J.E. Gould, J.W. Overstreet, H. Yanagimachi, R. Yanagimachi, D.F. Katz, and F.W. Hanson, What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs? Fertil. Steril. 40, 344 (1983).PubMedGoogle Scholar
  22. 22.
    In: “Research Frontiers in Fertility Regulation,” G.I. Zatuchni, ed., PARFR, Chicago (1985).Google Scholar
  23. 23.
    K.S.K. Tung, E.H. Goldberg, E. Goldberg, Immunobiological consequence of immunization of female mice with homologous spermatozoa: Induction of infertility, J. Reprod. Immunol. 1, 14 (1979).CrossRefGoogle Scholar
  24. 24.
    K.K. Ahuja, and S.J. Tzartos, Investigation of sperm receptors in the hamster zona pellucida by using univalent (Fab) antibodies to hamster ovary, J. Reprod. Fertil. 61, 257 (1981).PubMedCrossRefGoogle Scholar
  25. 25.
    R.J. Aitken, E. Rudak, D.W. Richardson, J. Dor, O. Djahanbakch, and A.A. Templeton, the influcence of antizona and anti-sperm antibodies on sperm-egg interactions, J. Reprod. Fertil. 62, 597 (1981).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Rajesh K. Naz
    • 1
  1. 1.Department of OB/GYNThe George Washington University Medical CenterUSA

Personalised recommendations