LHRH Immunoneutralization: Basic Studies and Prospects for Practical Application
The hypothalamic decepeptide, pG1u-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-G1y-NH2, luteinizing hormone releasing hormone (LHRH) or gonadotrophin releasing hormone, plays a central role in reproduction in all mammalian species. Our understanding of its physiological role has been guided on one hand by effects of administering LHRH at different doses and frequencies to humans or experimental animals who lack the hormone, and on the other by studying the consequences of desensitizing the pituitary by repeated exposure to agonist analogues, by blocking the LHRH receptors by chemical antagonists or by the neutralizing endogenous LHRH by antibodies (Fig. 1). These studies have shown that for continued stimulation, the pituitary gonadotroph must be exposed to small pulses of LHRH at a frequency of one per 60–120 min (1–3) which appear to the pattern of normal hypothalamic activity as reflected by the frequency of luteinizing hormone (LH) pulses from the pituitary gland and from what is known from measuring LHRH in the hypophysial portal blood (4).
KeywordsLuteinizing Hormone Follicle Stimulate Hormone Passive Immunization Luteinizing Hormone Release Hormone Gonadotrophin Secretion
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- 10.H.M. Fraser, ‘Effects of antibodies to luteinizing hormone releasing hormone on reproductive functions in rodents’, in: “Immunization with Hormones in Reproduction Research” E. Nieschlag, ed., North-Holland Publishing Company, Amsterdam (1975)Google Scholar
- 12.H.M. Fraser, Inhibition of reproductive function by antibodies to luteinizing hormone-releasing hormone, in: “Immunological aspects of reproduction and fertility control” J.P. Hearn, ed., MTP Press, Lancaster, U.K. (1980).Google Scholar
- 13.H.M. Fraser, R.M. Sharpe, G.A. Lincoln and A.J. Harmer, LHRH antibodies: Their use in the study of hypothalamic LHRH and testicular LHRH-like material, and possible contraceptive applications in: “Progress Towards a Male Contraceptive” S.L. Jeffcoate and M. Sandler eds., John Wiley & Sons Ltd. (1982).Google Scholar
- 14.H.M. Fraser, A.S. McNeilly and R.M. Popkin, Passive immunization against LHRH: elucidation of the role of LHRH in controlling LH and FSH secretion and LHRH receptors in: “Immunological Aspects of Reproduction in Mammals” B. Crighton ed., Butterworths (1984).Google Scholar
- 24.B.J. Keeling and D.B. Crighton, Reversibility of the effects of active immunization against LH-RH in: “Immunological Aspects of Reproduction in mammals” D.B. Crighton ed., Butterworths, London (1984).Google Scholar
- 36.H.M. Fraser, A.S. McNeilly, M. Abbott, W.R.A. Brown and R.A. Steiner, The effect of LHRH immunoneutralization on fillicular development, the LH surge and luteal function in the stumptailed macaque. J. Reprod. Fert (1985).Google Scholar
- 41.M. Takahashi, J.J. Ford, K. Yoshinaga and R.O. Greep, Active immunization of female rats with luteinizing hormone releasing hormone (LHRH) Biol. Reprod. 17: 754.Google Scholar
- 46.A.S. McNeilly, J.A. Jonassen and H.M. Fraser, Suppression of follicular development after long-term immunoneutralization of LHRH in the ewe. J. Reprod. Fert (submitted) (1985).Google Scholar
- 54.C. Das. Anti-GnRH monoclonals: pituitary and extrapituitary actions in control of fertility. This symposium.Google Scholar