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Structure and Regulated Transcription of DIRS-1, A Novel Dictyostelium Discoideum Transposable Element

  • Joe Cappello
  • Stephen M. Cohen
  • Karl Handelsman
  • Harvey F. Lodish
Part of the Stadler Genetics Symposia Series book series (SGSS)

Abstract

Transposons and transposon- and retrovirus-like DNA segments form a major part of the repetitive DNA in many eukaryotes. They are a cause of many “spontaneous” mutations in Drosophila and other organisms, and many catalyze the rearrangements of large segments of DNA that have occurred during evolution (Spradling and Rubin, 1981; Kleckner, 1981; Flavell et al., 1981; Roeder and Fink 1983; Fedoroff, 1983). In the course of cloning segments of Dictvostelium discoideum genomic DNA that are expressed preferentially during early differentiation, we isolated the DIRS-1 transposon (Chung et al., 1983; Zuker and Lodish, 1981). DIRS-1 is unusual in that its transcription is induced either by heat shock or by the high cell density that occurs during the early stages of differentiation. It is also novel in that its structure is unlike any transposon in prokaryotes or eukaryotes that has been identified to date. DIRS-1 has been independently isolated by Rosen et al. (1983) who call it Tdd-1.

Keywords

Heat Shock Transposable Element Terminal Repeat Dictyostelium Discoideum Inverted Terminal Repeat 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Joe Cappello
    • 1
    • 2
  • Stephen M. Cohen
    • 1
    • 2
  • Karl Handelsman
    • 1
    • 2
  • Harvey F. Lodish
    • 1
    • 2
  1. 1.Whitehead Institute for Biomedical ResearchNine Cambridge CenterCambridgeUSA
  2. 2.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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