Use of the Chemiluminigenic Probes Luminol and Lucigenin for the Detection of Active Oxygen Species in Hepatic Microsomes and in Intact Hepatocytes

  • Alfred G. Hildebrandt
  • Andreas Weimann
  • Regine Kahl
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 197)


During microsomal NADPH oxidation, reduced oxygen species are produced which must be considered a normal by-product of the NADPH-cytochrome P-450 reductase/cytochrome P-450 system (Gillette, Brodie and LaDu, 1957; Hildebrandt, Speck and Roots,1973; Estabrook, Kawano, Werringloer et al., 1979; Cederbaum and Dicker, 1983). Formation of such reactive oxygen molecules is enhanced in the presence of many monooxygenase substrates (Hildebrandt and Roots, 1975; Hildebrandt, IIeinemeyer and Roots, 1982) and may contribute to the toxicity of foreign compounds undergoing microsomal biotransformation ( Farber and Gerson, 1984 ). It has been suggested that superoxide anion radicals are released during autoxidation of the oxycytochrome P-450 complex and are subsequently dismutated to yield hydrogen peroxide ( Ullrich and Kuthan, 1980 ); hydroxyl radical formation may then result from hydrogen peroxide cleavage in the presence of superoxide anion by a Haber-Weiss reaction ( Haber and Weiss, 1934 ).


Butylate Hydroxy Toluene Propyl Gallate Butylate Hydroxyanisole Propyl Gallate Photon Yield 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Alfred G. Hildebrandt
    • 1
  • Andreas Weimann
    • 1
  • Regine Kahl
    • 1
  1. 1.Max von Pettenkofer Institute of the Federal HealthBerlin 33Germany

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