Inhibition of Respiration in Rabbit Proximal Tubules by Bromophenols and 2-Bromohydroquinone
The kidney cortex is a highly aerobic and metabolically active tissue rich in mitochondria and Na-and K-dependent adenosine triphosphatase (Na,K-ATPase) activity. The basal respiration rate of a suspension of rabbit proximal tubules utilizes 50-60 percent of the mitochondrial respiratory capacity (Harris et al., 1981). Since Na,K-ATPase-mediated ion transport consumes more metabolic energy than any other single enzymatic process within the mammalian organism, it is not surprising that rabbit proximal tubules utilize half of their basal respiration for this process (Cohen and Kamm, 1976; Harris et al., 1981). The remaining half of their basal respiration is divided between ATP generation for other processes and nonphosphorylating respiration. Using isolated mitochondria, nonphosphorylating respiration has been shown to be 5–8 percent of the basal respiration (Davis et al., 1974).
KeywordsProximal Tubule Mitochondrial Respiration Sodium Butyrate Basal Respiration Renal Proximal Tubule
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