Inhibition of Respiration in Rabbit Proximal Tubules by Bromophenols and 2-Bromohydroquinone

  • Rick G. Schnellmann
  • Lazaro J. Mandel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 197)


The kidney cortex is a highly aerobic and metabolically active tissue rich in mitochondria and Na-and K-dependent adenosine triphosphatase (Na,K-ATPase) activity. The basal respiration rate of a suspension of rabbit proximal tubules utilizes 50-60 percent of the mitochondrial respiratory capacity (Harris et al., 1981). Since Na,K-ATPase-mediated ion transport consumes more metabolic energy than any other single enzymatic process within the mammalian organism, it is not surprising that rabbit proximal tubules utilize half of their basal respiration for this process (Cohen and Kamm, 1976; Harris et al., 1981). The remaining half of their basal respiration is divided between ATP generation for other processes and nonphosphorylating respiration. Using isolated mitochondria, nonphosphorylating respiration has been shown to be 5–8 percent of the basal respiration (Davis et al., 1974).


Proximal Tubule Mitochondrial Respiration Sodium Butyrate Basal Respiration Renal Proximal Tubule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bergmeyer, H.-U., Bernt, E., and Hess, B., 1973, Lactic dehydrogenase, in: “Methods of Enzymatic Analysis,” H.-U. Bergmeyer, ed., Academic Press, London.Google Scholar
  2. Chance, B. and Williams, G.R., 1956, The respiratory chain and oxidative phosphorylation. Adv. Enzymol., 17: 65.Google Scholar
  3. Cohen, J.J. and Kamm, D.E., 1976, Renal metabolism: relation to renal function, in: “The Kidney,” B.M. Brenner and F.C. Rector, eds., Saunders, Philadelphia.Google Scholar
  4. Davis, J.E., Lumeng, L., and Bottoms, D., 1974, On the relationships between the stoichiometry of oxidative phosphorylation and the phosphorylation potential of rat liver mitochondria as functions of respiratory rate, FEBS Lett., 39: 9.PubMedCrossRefGoogle Scholar
  5. Harris, S.I., Balaban, R.S., Barrett, L., and Mandel, L.J., 1981, Mito-chondrial respiratory capacity and Na+- and K+-dependent adenonsine triphosphatase-mediated ion transport in the intact renal cell, J. Biol. Chem., 256: 10319.PubMedGoogle Scholar
  6. Gornall, A.G., Bardawill, C.J., and David, M.M., 1949, Determination of serum proteins by means of a biuret reaction, J. Biol. Chem., 177: 751.PubMedGoogle Scholar
  7. Lau, S.S., Monks, T.J., and Gillette, J.R., 1984, Identification of 2bromohydroquinone as a metabolite of bromobenzene and o-bromophenol: Implications for bromobenzene-induced nephrotoxicity, J. Pharmacol. Exp. Ther., 230: 360.PubMedGoogle Scholar
  8. Schnellmann, R.G. and Mandel, L.J., 1985a, Multiple effects of presumed glutathione depletors on rabbit proximal tubules, Kidney Int. (in press)Google Scholar
  9. Schnellmann, R.G. and Mandel, L.J., 1985b, Cellular toxicity of bromobenzene and bromobenzene metabolites to rabbit proximal tubules: The role and mechanism of 2-bromohydroquinone, J. Pharmacol. Exp. Ther. (submitted).Google Scholar
  10. Soltoff, S.P. and Mandel, L.J., 1984, Active ion transport in the renal proximal tubule, J. Gen. Physiol., 84: 601.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Rick G. Schnellmann
    • 1
  • Lazaro J. Mandel
    • 1
  1. 1.Department of PhysiologyDuke University Medical CenterDurhamUSA

Personalised recommendations