Deoxyguanosine Adducts Formed from Benzoquinone and Hydroquinone
The mechanism by which benzene produces bone marrow damage is not known with certainty, but it is generally accepted that the toxicity is mediated by one or more benzene metabolites. Radiolabeled metabolites of benzene administered in vivo have been shown to covalently bind to DNA in rat liver (Lutz and Schlatter, 1977) and mouse bone marrow (Gill and Ahmed, 1981). Rabbit bone marrow mitoplasts have been found to metabolize benzene to products which bind to mitochondria) DNA (Rushmore, et al., 1984). Hydrolysis of the DNA to nucleosides yielded 6–7 possible adducts to guanosine. It is hypothesized that the covalent binding of benzene metabolites to DNA may be an etiological factor in benzene-induced bone marrow depression.
KeywordsPotassium Phosphate Buffer Aplastic Anemia Covalent Binding Mouse Bone Marrow Benzene Metabolite
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