Induction of Unscheduled DNA Synthesis in Human Mononuclear Leukocytes by Oxidative Stress

  • Ralf Morgenstern
  • Ronald W. Pero
  • Daniel G. Miller
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 197)


The effect on unscheduled DNA synthesis (UDS) of different agents that induce oxidative stress was investigated using human mononuclear leukocytes (HML). It was found that Xanthine plus Xanthine oxidase increaseed UDS by 47%. Hydrogen peroxide had no significant effect. Cumene hydroperoxide increases UDS by 50% with a large interindividual variation (7–100%). Bleomycin and Mitomycin C increase UDS 189% and 295%, respectively. The validity of UDS induced by these agents for screening interindividual differences in susceptibility to oxidative stress is discussed.


Xanthine Oxidase Ataxia Telangiectasia Cumene Hydroperoxide Large Interindividual Variation Xanthine Oxidase System 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Ralf Morgenstern
    • 1
  • Ronald W. Pero
    • 1
  • Daniel G. Miller
    • 1
  1. 1.Preventive Medicine Institute-Strang ClinicNew YorkUSA

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