The Role of Reactive Intermediates in Sulfhydryl-Dependent Immunotoxicity: Interference with Microtubule Assembly and Microtubule-dependent Cell Function
The critical role of protein and low molecular weight thiols in cell homeostasis has long been appreciated. Sulfhydryl(SH) reagents have been shown to interfere with numerous biochemical processes critical for cell integrity including numerous SH-dependent enzymes, protein, RNA and DNA synthesis and in the maintenance of normal intracellular redox potential. On a different level, the lymphocyte appears to be unusually sensitive to certain SH-reagents at concentrations that do not result in non-specific cytotoxicity or cell death. SH reagents, for example, are potent suppressors of normal immune cell function via mechanisms that apparently are distinct from cytotoxic events frequently associated with the exposure to SH-reactive intermediates. Both surface and intracellular SH groups have been shown to be involved in lymphocyte activation and maintenance of normal cell function, however, this discussion will be restricted largely to membrane penetrating SH-reagents.
KeywordsMicrotubule Assembly Sulfhydryl Reagent Lymphocyte Blastogenesis Blastogenic Response Quinone Metabolite
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