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Formation and Identification of Naphthoquinone Glutathione Conjugates Following Microsomal Metabolism of 1-Naphthol

  • Marion G. Miller
  • John Powell
  • Gerald M. Cohen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 197)

Abstract

Previous studies have indicated that 1-naphthol is metabolised by the cytochrome P-450 mixed function oxidase enzyme system to form a reactive species capable of covalently binding to microsomal protein (1,2). Furthermore, Hesse and Mezger (1) suggested the involvement of quinones and/or semiquinones in 1-naphthol-dependent covalent binding. In more recent studies, 1,4-naphthoquinone formed from 1-naphthol has been directly measured with both rat liver microsomes (2,3) and a reconstituted cytochrome P-450 enzyme system (4). From this, it was hypothesised that the toxicity of 1-naphthol, previously reported in isolated hepatocytes (5), may be mediated by naphthoquinone metabolites of l-naphthol.

Keywords

Covalent Binding Fast Atom Bombardment Microsomal Protein Proton Nuclear Magnetic Resonance Glutathione Conjugate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Marion G. Miller
    • 1
  • John Powell
    • 2
  • Gerald M. Cohen
    • 1
  1. 1.The Toxicology Unit The School of PharmacyUniversity of LondonLondonUK
  2. 2.Department of Pharmaceutical Chemistry, The School of PharmacyUniversity of LondonLondonUK

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