Analysis of Hormone Secretion from Individual Pituitary Cells
Analysis of the function of individual neurons and immune cells (B lymphocytes) has been enormously profitable for the understanding of the nervous and immune systems. Indeed, specialization of individual cells is central to theories underlying the function of those systems. In contrast, homogeneity among cells subserving various functions in endocrine and exocrine secretory systems is the common assumption. These differences in viewpoint may arise simply from the availability of methods for the study of individual neurons (single unit electrical recordings) and immune cells (hemolytic plaque assay for detection of antibody secretion) on the one hand, and their absence for individual endocrine and exocrine cells, on the other. Thus, the extent to which heterogeneity in cell function contributes to the function of endocrine and exocrine systems is unknown.
KeywordsPituitary Cell Plaque Assay GnRH Receptor Plaque Area Total Plaque Area
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- Hymer, W.C., and Hatfield, J.M., 1983, Separation of cells from the rat anterior pituitary gland, in “Methods in Enzymology”, P.M. Conn, ed., Academic Press, New York, 103:257.Google Scholar
- Smith, M.S., Freeman, M.E., and Neill, J.D., 1975, The control of progesterone secretion during the estrous cycle and early pseudopregnancy in the rat: prolactin, gonadotropin, and steroid levels associated with rescue of the corpus luteum of pseudopregnancy, Endocrinology 96:219.PubMedCrossRefGoogle Scholar
- Smith, P.F., Luque, E.H., and Neill, J.D., 1985, Detection and measurement of secretion from individual neuroendocrine cells using a reverse hemolytic plaque assay, in “Methods in Enzymology”, P.M. Conn, ed., Academic Press, New York (in press).Google Scholar
- Smith, P.F., and Neill, J.D., 1983, Heterogeneity in secretory response to GnRH within the rat pituitary gonadotrope population: quantitative analysis at the single cell level, Program of the 13th Annual Meeting of the Society for Neuroscience, Boston, MA, p. 17.Google Scholar
- Smith, P.F., and Neill, J.D., 1984, The reverse hemolytic plaque assay for detection of hormone secretion by individual cells, in “Excerpta Medica International Congress Series (7th International Congress of Endocrinology)” F. Labrie and L. Proulx, eds., Elsevier Science Publishers, Amsterdam, pp. 1113–1116.Google Scholar