Hormonal Regulation of Pituitary Gonadotropin Gene Expression

  • R. Counis
  • M. Corbani
  • A. Starzec
  • M. Jutisz
Part of the Biochemical Endocrinology book series (BIOEND)


Lutropin (LH) and follitropin (FSH) are two members of a family of structurally related polypeptide hormones, that also includes pituitary thyrotropin (TSH) and placental human chorionic gonadotropin (hCG). Each hormone of this family contains two non identical, non covalently linked subunits, α and β. The primary structure of the a subunits is identical among hormones within a species, whereas the primary structures of the β subunits differ greatly and confer to the hormones their specific biological activities (Pierce and Parsons, 1981). Although they are not identical, the β subunits show enough homology in their primary structures to suggest that they arose by duplications and mutations of a single ancestral gene (Fontaine and Burzawa-Gérard, 1977).


Luteinizing Hormone Pituitary Cell Gonadal Steroid Protein Synthesise System Cell Free Synthesis 
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  1. Alexander, D.C., and Miller, W.L., 1981, mRNA for ovine follicle-stimulating hormone β-chains; an in vitro translational assay, J. Biol. Chem., 25b: 12628–12631.Google Scholar
  2. Alexander, D.C., and Miller, W.L., 1982, Regulation of ovine follicle-stimulating hormone β-chain mRNA by 17β-estradiol in vivo and in vitro, J. Biol. Chem., 257:2282–2286.PubMedGoogle Scholar
  3. Azhar, S., Reel, J.R., Pastushok, C.A., and Menon, K.M.J., 1978, LH biosynthesis and secretion in rat anterior pituitary cell cultures: stimulation of LH glycosylation and secretion by GnRH and an agonistic analogue and blockade by an antagonistic analogue, Biochem. Biophys. Res. Commun., 80:659–666.PubMedCrossRefGoogle Scholar
  4. Bérault, A., Théoleyre, M., Colleaux, Y., and Jutisz, M., 1980, Further evidence that cyclic AMP is not the mediator of the releasing action of GnRH, Neuroendocr. Letters, 2:31–37.Google Scholar
  5. Chin, W.W. and Habener, J.F., 1984, Organization and expression of lutropin (LH) subunit genes, 7th Int. Congress of Endocrinology, Quebec City, Canada, Abstr. no S 47.Google Scholar
  6. Corbani, M., Counis, R., Starzec, A., and Jutisz, M., 1980, Effect of gonadectomy on pituitary levels of mRNA encoding gonadotropin subunits and secretion of luteinizing hormone, Mol. Cell. Endocr., 35:83–87.CrossRefGoogle Scholar
  7. Counis, R., Ribot, C., Corbani, M., Poissonnier, M., and Jutisz, M., 1981, Cell-free translation of the rat pituitary messenger RNA coding for the precursors of α-and β-subunits of lutropin, FEBS Letters, 123:151–155.PubMedCrossRefGoogle Scholar
  8. Counis, R., Corbani, M., Ribot, C., and Jutisz, M., 1982a, Characterization of the precursors of α-and β-subunits of follitropin following cell-free translation of rat and ovine pituitary mRNAs, Biochem Biophys. Res. Commun., 107:998–1005.PubMedCrossRefGoogle Scholar
  9. Counis, R., Corbani, M., and Jutisz, M., 1982b, Studies on cell-free biosynthesis of lutropin (LH) and characterization of its subunit precursors, in: “Pituitary hormones and related peptides”, M. Motta, M. Zanisi, and F. Piva, eds., Academic Press, London and New-York, pp 49–61.Google Scholar
  10. Counis, R., Corbani, M., and Jutisz, M., 1982c, Régulation de la biosynthèse des gonadotropines hypophysaires, in: “Régulations cellulaires multihormonales en Neuroendocrinologie”, A. Tixier-Vidal and Ph. Richard, eds., Colloques de l’INSERM, Paris, 110: 509–524.Google Scholar
  11. Counis, R., Corbani, M., and Jutisz, M., 1983, Estradiol regulates mRNAs encoding precursors to rat lutropin (LH) and follitropin (FSH) subunits, Biochem. Biophys. Res. Commun., 114:65–72.PubMedCrossRefGoogle Scholar
  12. Counis, R., Corbani, M., and Jutisz, M., 1984, In vivo regulation by estradiol of the messenger RNAs encoding LH and FSH subunits and the secretion of gonadotropins, in: “Hormonal control of the hypothalamo-pituitary-gonadal axis”, K.W. McKerns and Z. Naor, eds., Plenum Press, pp. 397-410.Google Scholar
  13. Fiddes, J.C., and Goodman, H.M., 1979, Isolation, cloning and sequence analysis of the cDNA for the α-subunit of human chorionic gonadotropin, Nature, 281:351–356.PubMedCrossRefGoogle Scholar
  14. Fiddes, J.C., and Goodman, H.M., 1980, The cDNA for the β-subunit of human chorionic gonadotropin suggests evolution of a gene by readthrough into the 3′-untranslated region, Nature, 286:684–687.PubMedCrossRefGoogle Scholar
  15. Fontaine, Y.A., and Burzawa-Gérard, E., 1977, Esquisse de l’évolution des hormones gonadotropes et thyréotropes des vertébrés, Gen. Comp. Endocr., 32:341–347.PubMedCrossRefGoogle Scholar
  16. Franchimont, P., and Pasteeis, J.L., 1972, Sécrétion indépendante des hormones gonadotropes et de leurs sous-unités, C.R. Acad. Sci. Paris, Série D, 275: 1799–1802.Google Scholar
  17. Grotjan, H.E. Jr., Leveque, N.W., Berkowitz, A.S., and Keel, B.A., 1984, Quantitation of LH subunits released by rat anterior pituitary cells in primary culture, Mol. Cell. Endocr., 35:121–129.CrossRefGoogle Scholar
  18. Hagen, C., and McNeilly, A.S., 1975, Changes in circulating levels of LH, FSH, LHβ and α-subunit after gonadotropin-releasing hormone, and of TSH, LHβ and α-subunits after thyrotropin-releasing hormone, J. Clin. Endocrinol. Metab., 41:466–470.PubMedCrossRefGoogle Scholar
  19. Keller, D., Fetherson, J., and Boime, I., 1980, Isolation of mRNA from bovine pituitary: the cell free synthesis of the α-and β-subunits of luteinizing hormone, Eur. J. Biochem., 108:367–372.PubMedCrossRefGoogle Scholar
  20. Khar, A., and Jutisz, M., 1980, Role of gonadotropin releasing hormone in the biosynthesis of LH and FSH by rat anterior primary cells in culture, in: “Synthesis and release of adenohypophyseal hormones”, M. Jutisz and K.W. McKerns, eds., Plenum Press, New York and London, pp. 217–235.Google Scholar
  21. Khar, A., Debeljuk, L., and Jutisz M., 1978, Biosynthesis of gonadotropin by rat pituitary cells in culture and in pituitary homogenates: effect of gonadotropin-releasing hormone, Mol. Cell. Endocr., 12:53–65.CrossRefGoogle Scholar
  22. Landefeld, T.D., 1979, Identification of in vitro synthesized pituitary glycoprotein a subunit. Translation of a possible precursor, J. Biol. Chem., 254:3585–3688.Google Scholar
  23. Landefeld, T.D., and Kepa, J., 1979, The cell free synthesis of bovine lutropin β-subunit, Biochem. Biophys. Res. Commun., 90:1111–1118.PubMedCrossRefGoogle Scholar
  24. Landefeld, T.D., Kepa, J., and Karsh, F.J., 1983, Regulation of α-subunit synthesis by gonadal steroid feedback in the sheep anterior pituitary, J. Biol. Chem., 258:2390–2393.PubMedGoogle Scholar
  25. Landefeld, T.D., Kepa, J., and Karsh, F., 1984, Estradiol feedback effects on the α-subunit mRNA in the sheep pituitary gland: correlation with serum and pituitary hormone concentrations, Proc. Natl. Acad. Sci. USA, 81: 1322–1326.PubMedCrossRefGoogle Scholar
  26. Liu, T.C., and Jackson, G.L., 1978, Modifications of luteinizing hormone biosynthesis and release by GnRH, cycloheximide and antinomycin D, Endocrinology, 103:1253–1263.PubMedCrossRefGoogle Scholar
  27. Liu, T.C., and Jackson, G.L., 1985, Synthesis and release of LH in vitro: effects of gallopamil hydrochloride (D600) and pimozide, 67th Annual Meeting of the Endocrine Society, Baltimore, Abstr. no 423.Google Scholar
  28. Liu, T.C., Jackson, G.L., and Gorski, J., 1976, Effects of synthetic gonadotropin-releasing hormone on incorporation of radioactive glucosamine and amino acids into luteinizing hormone and total protein by rat pituitaries in vitro, Endocrinology, 98:151–163.PubMedCrossRefGoogle Scholar
  29. McCann, S.M., and Ramirez, V.D., 1964, The neuroendocrine regulation of hypophyseal luteinizing hormone secretion; Recent Progr. Horm. Res., 20:131–181.Google Scholar
  30. Nilson, J.H., Nejedlik, M.T., Virgin, J.B., Crowder, M.E., and Nett, T.M., 1983, Expression of α-subunit and luteinizing hormone β-genes in the ovine anterior pituitary; estradiol supresses accumulation of mRNAs for both α-subunit and luteinizing hormone-β, J. Biol. Chem., 258:12087–12090.PubMedGoogle Scholar
  31. Parsons, T.C., and Pierce, J.G., 1984, Free α-like material from bovine pituitaries, J. Biol. Chem., 259:2662–2666.PubMedGoogle Scholar
  32. Pierce, J.G., and Parsons, T.F., 1981, Glycoprotein hormones: structure and function, Ann. Rev. Biochem., 50:465–495.PubMedCrossRefGoogle Scholar
  33. Schally, A.V., Kastin, A.J., and Arimura, A., 1972, FSH-releasing hormone and LH-releasing hormone, Vitamins and Hormones, 30:83–164.PubMedCrossRefGoogle Scholar
  34. Vogel, D.L., Mayner, J.A., and Sherins, R.J., 1983, Biosynthesis of LH-alpha and-beta subunits in intact and orchiectomized rats, 65th Annual Meeting of the Endocrine Society, San Antonio, Abstr. no 144.Google Scholar
  35. Wallace, R.B., Shaffer, J., Murphy, R.F., Bonner, J., Hirose, T., and Itakura, K., 1979, Hybridization of synthetic oligodeoxynucleotides to 0x 174 DNA: the effect of single base pair mismatch, Nucleic Acids Res., 6:3543–3557.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • R. Counis
    • 1
  • M. Corbani
    • 1
  • A. Starzec
    • 1
  • M. Jutisz
    • 1
  1. 1.Laboratoire des Hormones PolypeptidiquesCNRSGif sur YvetteFrance

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