Localization of Nucleic Acids in Sections

  • H. C. Aldrich
  • Sylvia E. Coleman


Occasionally, structures of unknown composition are observed inside the nucleus or cytoplasm of eukaryotic microbes or in the cytoplasm of prokaryotes. If the structure is suspected to contain DNA or RNA, there is a varied arsenal of techniques that will aid in identification. Nucleic acids can be detected by staining methods that utilize heavy metals, by labeling, and by extraction. We will review the most useful of these techniques, emphasizing procedures that can be used on ultrathin sections.


Uranyl Acetate Lead Citrate Glycol Methacrylate Colloidal Gold Mixed Connective Tissue Disease 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • H. C. Aldrich
    • 1
  • Sylvia E. Coleman
    • 2
  1. 1.Department of Microbiology and Cell ScienceUniversity of FloridaGainesvilleUSA
  2. 2.Research ServiceVeterans Administration Medical CenterGainesvilleUSA

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