Gene Transfer into Animals by Retroviral Vectors
In a previous chapter (W.F. Anderson, this Volume), there was a dis-cussion of why the unique life cycle of retroviruses makes them attractive candidates for use as agents for gene transfer into animals. However, a number of specific requirements need to be met (1). In order to be useful, a potential vector should be generated at a sufficiently high titer so that the infection of such rare target cells as the hematopoietic stem cells would be likely. The vector should become integrated as a stable, intact sequence, not only in cultured cells, but also in whole animals. Finally, expression of transferred genes in animals should be at or near physiological levels in order for such gene transfer to have potential practical applicability.
KeywordsBone Marrow Cell Murine Leukemia Virus Human Gene Therapy Proviral Sequence Phosphotransferase Activity
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