Compartmentation of Adenine Nucleotides and Phosphocreatine Shuttle in Cardiac Cells: Some New Evidence

  • Valdur A. Saks
  • A. V. Kuznetsov
  • Z. A. Huchua
  • V. V. Kupriyanov
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 194)


The concept of the phosphocreatine shuttle for intracellular energy channelling in muscle cells (1 — 6) assumes compartmentation of adenine nucleotides in mitochondrial and myofibrillar compartments and around membranes. The link between these compartments is considered to be performed by phosphocreatine molecules due to heterogeneous distribution of creatine kinase isoenzymes functioning in mitochondria in direction of creatine phosphorylation and in myofibrils in direction of phosphocreatine dephosphorylation. While different isoenzymes of creatine kinase are well characterized, adenine nucleotide compartmentation still remains to be an unsolved question. In our recent studies described below a competitive enzyme method was used to show the existence of rather closed functional cycles of adenine nucleotides in mitochondria and in myofibrils due to titivation of creatine kinase in those structures. Also, 31P-NMR saturation transfer method was used to show the role of those coupled creatine kinases inside mitochondrial and myofibrillar compartments in the integrated intracellular energy fluxes in isolated perfused rat hearts at different workloads.


Creatine Kinase Oxidative Phosphorylation Pyruvate Kinase Adenine Nucleotide Creatine Kinase Isoenzyme 


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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Valdur A. Saks
    • 1
  • A. V. Kuznetsov
    • 1
  • Z. A. Huchua
    • 1
  • V. V. Kupriyanov
    • 1
  1. 1.BioenergeticsUSSR Research Center for CardiologyMoscowRussia

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