The Effect of Inorganic Phosphate on Mitochondrial, Creatine Kinase

  • Norman Hall
  • Marlene DeLuca
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 194)


Since the last meeting at Johns Hopkins in 1979, there has been a continued interest and a great deal of progress made in our understanding of the role of mitochondrial creatine kinase in muscle cells. The existence of the creatine phosphate shuttle as discussed by Bessman and Geiger (1) is now known to be an important aspect of muscle metabolism. There have been numerous studies on the effect of creatine kinase on respiration in heart muscle mitochondria. It is now well-documented that mitochondrial creatine kinase is responsible for stimulating respiration by utilizing ATP produced by oxidative phosphorylation and maintaining a constant high level of ADP available for continued phosphorylation. There has been much discussion about whether the ATP produced by oxidative phosphorylation is preferentially used by the mitochondrial creatine kinase. This aspect will be addressed by many of the other speakers here.


Creatine Kinase Liver Mitochondrion Creatine Kinase Activity Heart Mitochondrion Breast Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    S.P. Bessman and P.J. Geiger, Transport of energy in muscle: The phosphorylcreatine shuttle, Science 211: 448 (1981).Google Scholar
  2. H.E. Blum, B. Deus, and W. Gerok, Mitochondrial creatine kinase from human heart muscle: Purification and characterization of the crystallized isoenzyme, J. Biochem. 94:1247 (1983).Google Scholar
  3. 3.
    N. Hall, P. Addis, and M. DeLuca, Mitochondrial creatine kinase. Physical and kinetic properties of the purified enzyme from beef heart. Biochemistry 18: 1745 (1979).PubMedCrossRefGoogle Scholar
  4. 4.
    V.A. Saks, G.B. Chernousova, I.I. Voronkor, U.N. Smirnov, and E.I. Chazov, Study of energy transport mechanism in myocardial cells, Circ. Res. (Suppl. III) 34–35: 138 (1974).Google Scholar
  5. 5.
    N. Hall and M. DeLuca, unpublished observations.Google Scholar
  6. 6.
    E.C. Farrell, N. Baba, G.P. Brierly, and H-D. Grumer, On the creatine phosphokinase of heart muscle mitochondria, Lab Invest. 27: 209 (1972).Google Scholar
  7. H.K. Jacobs and M. Graham, Physical and chemical characterization of mitochondrial creatine kinase from bovine heart, Fed. Proc. 37:1574 (1978).Google Scholar
  8. R. Roberts and A.M. Grace, Purification of mitochondrial creatine kinase. Biochemical and immunological characterization, J. Biol. Chem. 255:2870 (1980).Google Scholar
  9. R.A. Wevers, C.P.M. Reutelingsperger, B. Dam, and J.B.J. Soons, Mitochondrial creatine kinase in the brain, Clin. Chim. Acta 119:209 (1981).CrossRefGoogle Scholar
  10. F. Kanemitsu, I. Kawanishi, and J. Mizushima, Characteristics of mitochondrial creatine kinases from normal human heart and liver tissues, Clin. Chim. Acta 119:307 (1982).Google Scholar
  11. 11.
    W.E. Jacobus, J.A. Bittl, and M.L. Weisfeldt, Loss of mitochondrial creatine kinase in vitro and in vivo: A sensitive index of ischemic cellular and functional damage, in: “Heart Creatine Kinase, the Integration of Isozymes for Energy Distribution,” W.E. Jacobus and J.S. Ingwall, eds., Williams and Wilkins, Baltimore (1980).Google Scholar
  12. C. Vial, B. Font, D. Goldschmidt, and D.C. Gautheron, Dissociation and reassociation of creatine kinase with heart mitochondria; pH and phosphate dependence, Biochem. Biophys. Res. Comm. 88:1352 (1979).PubMedCrossRefGoogle Scholar
  13. N. Hall and M. DeLuca, Binding of creatine kinase to heart and liver mitochondria in vitro, Arch. Biochem. Biophys. 201:674 (1980).PubMedCrossRefGoogle Scholar
  14. U.A. Saks, V.V. Kupriyanov, G.V. Elizarova, and W.E. Jacobus, Studies of energy transport in heart cells: The importance of creatine kinase localization for the coupling of mitochondrial phosphorylcreatine production to oxidative phosphorylation, J. Biol. Chem. 255:755 (1980).PubMedGoogle Scholar
  15. 15.
    N. Hall and M. DeLuca, The effect of inorganic phosphate on creatine kinase in respiring rat heart mitochondria, Arch. Biochem. Biophys. (1984) in press.Google Scholar
  16. W.E. Jacobus and V.A. Saks, Creatine kinase of heart mitochondria: Changes in its kinetic properties induced by coupling to oxidative phosphorylation, Arch. Biochem. Biophys. 219: 167 (1982).Google Scholar
  17. U.D. Bennett, N. Hall, M. DeLuca, and C.H. Suelter, Decreased mitochondrial creatine kinase activity alters the function of the creatine phosphate shuttle in dystrophic chicken breast muscle, submitted to J. Biol. Chem. (1984).Google Scholar
  18. M. DeLuca, N. Hall, R. Rice, and N.O. Kaplan, Creatine kinase isozymes in human tumors, Biochem. Biophys. Res. Comm. 99:189 (1981).PubMedCrossRefGoogle Scholar
  19. 19.
    T.Y. Lipskaya, V.D. Templ, L.V. Belovsova, E.U. Molokova, and I.V. Rybina, Investigation of the interaction of mitochondrial creatine kinase with the membranes of the mitochondria, Biochemistry - New York (translation of Biokhimiya) 45: 877 (1980).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Norman Hall
    • 1
  • Marlene DeLuca
    • 1
  1. 1.Departments of Medicine and ChemistryUniversity of California, San DiegoLa JollaUSA

Personalised recommendations