Calcium Compartmentation in Mammalian Myocardium

  • G. A. Langer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 194)


Since I have written recently published (Langer et al., 1982) or soon to be published (Langer, 1984) reviews on various aspects of calcium (Ca) compartmentation this report will serve as a brief summary of my present concepts of compartmentation with a focus on recent work from the Cardiovascular Research Laboratory at UCLA. These studies support the concept that Ca is distributed on and within the cell very differently dependent upon conditions of perfusion and functional demands.


Sarcoplasmic Reticulum Contractile Force Sarcolemmal Membrane Sarcolemmal Vesicle Diaminobutyric Acid 


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  1. Bers, D.M., and Langer, G.A., 1979, Uncoupling cation effects on cardiac contractility and sarcolemmal Ca2+ binding. Am. J. Physiol. 237: H332 - H341PubMedGoogle Scholar
  2. Bers, D.M., Philipson, K.D, and Langer, G.A., 1981, Cardiac contractility and sarcolemmal calcium binding in several cardiac muscle preparations. Am. J. Physiol. 240: H576 - H583PubMedGoogle Scholar
  3. Burt, J.M., and Langer, G.A., 1983, Ca2+ displacement by Polymyxin B from sarcolemma isolated by “gas dissection” from cultured neonatal rat myocardial cells. Biochim. Biophys. Acta 729: 44–52PubMedCrossRefGoogle Scholar
  4. Burt, J.M., Duenas, C.J., and Langer, G.A., 1983, Influence of Polymyxin B, a probe for anionic phospholipids, on calcium binding and calcium and potassium fluxes of cultured cardiac cells. Circ. Res. 53: 679–687PubMedGoogle Scholar
  5. Caroni, P., and Carafoli, E., 1981, The Ca2+-pumping ATPase of heart sarcolemma. Characterization, calmodulin dependence, and partial purification. J. Biol. Chem. 256: 3263–3270Google Scholar
  6. Fabiato, A., 1983, Calcium-induced release of calcium from the cardiac sarcoplasmic reticulum. Am. J. Physiol. 245: C1 - C14PubMedGoogle Scholar
  7. Fabiato, A., and Fabiato, F., 1978, Calcium-induced release of calcium from the sarcoplasmic reticulum of skinned cells from adult human, dog, cat, rabbit, rat and frog hearts and from fetal and new-born rat ventricles. Am. J. Physiol. 307: 491–522Google Scholar
  8. Langer, G.A., Frank, J.S., Nudd, L.M., and Seraydarian, K., 1976, Sialic acid: Effect of removal on calcium exchangeability of cultured heart cells. Science 193: 1013–1015PubMedCrossRefGoogle Scholar
  9. Langer, G.A., and Nudd, L.M., 1980, Addition and kinetic characterization of mitochondrial calcium in myocardial tissue culture. Am. J. Physiol. 239: H769 - H774PubMedGoogle Scholar
  10. Langer, G.A., Frank, J.S., and Philipson, K.D., 1981, Correlation of alterations in cation exchange and sarcolemmal ultrastructure produced by neuraminidase and phospholipases in cardiac cell culture. Circ. Res. 49: 1289–1299PubMedGoogle Scholar
  11. Langer, G.A., Frank, J.S., and Philipson, K.D., 1982, Ultrastr uc -ture and calcium exchange of the sarcolemma, sarcoplasmic reticulum and mitochondria of the myocardium. Pharmac. Therap. 16: 331–376CrossRefGoogle Scholar
  12. Langer, G.A., and Nudd, L.M., 1983, Effects of cations, phospholipases and neuraminidase on calcium binding to “gas dissected” membranes from cultured cardiac cells. Circ. Res. 53: 482–490PubMedGoogle Scholar
  13. Langer, G.A., Rich, T.L., and Nudd, L.M., 1983, Calcium compartmentalization in cultured and adult myocardium: Activation of a caffeine-sensitive component. J. Mol. Cell. Cardiol. 15: 459–473PubMedCrossRefGoogle Scholar
  14. Langer, G.A., 1984, Calcium at the sarcolemma. J. Mol. Cell. Cardiol. 16: In pressGoogle Scholar
  15. Lehninger, A.L., 1974, Role of phosphate and other proton-donating anions in respiration-coupled transport of Ca2+ by mitochondria. Proc. Natl. Acad. Sci. 71: 1520–1524PubMedCrossRefGoogle Scholar
  16. Lüllman, H., and Peters, T., 1977, Plasmalemmal calcium in cardiac excitation-contraction coupling. Clin. Exp. Pharmacol. Physiol. 4: 49–57CrossRefGoogle Scholar
  17. Philipson, K.D., and Langer, G.A., 1979, Sarcolemmal bound calcium and contractility in the mammalian myocardium. J. Mol. Cell. Cardiol. 11: 857–875PubMedCrossRefGoogle Scholar
  18. Philipson, K.D., Bers, D.M., and Nishimoto, A.Y., 1980a, The role of phospholipids in the Ca2+ binding of isolated cardiac sarcolemma. J. Mol. Cell. Cardiol. 12: 1159–1173PubMedCrossRefGoogle Scholar
  19. Philipson, K.D., Bers, D.M. Nishimoto, A.Y., and Langer, G.A., 1980b, Binding of Ca2+ and Na+ to sarcolemmal membranes: relation to control of myocardial contractility. Am. J. Physiol. 238: H373 - H378Google Scholar
  20. Philipson, K.D., and Nishimoto, A.Y., 1984, Stimulation of Nat-Ca2+ exchange in cardiac sarcolemmal vesicles by phospholipase D. J. Biol. Chem. In pressGoogle Scholar
  21. Ponce-Pornos, J.E., and Langer, G.A., 1982, Effects of inorganic phosphate on ion exchange, energy state, and contraction in mammalian heart. Am. J. Physiol. 242: H79 - H88Google Scholar
  22. Ponce-Hornos, J.E., Langer, G.A., and Nudd, L.M., 1982, Inorganic phosphate: its effects on Ca exchange and compartmentalization in cultured heart cells. J. Mol. Cell. Cardiol. 14: 41–51PubMedCrossRefGoogle Scholar
  23. Robertson S.P., Potter, J.D., and Rouslin, W., 1982, The Ca2+ and Mg2+ dependence of Ca2+ uptake and respiratory function of porcine heart mitochondria. J. Biol. Chem. 257: 1743–1748PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • G. A. Langer
    • 1
  1. 1.Departments of Medicine and Physiology Cardiovascular Research LaboratoriesUCLA School of Medicine Center for the Health SciencesLos AngelesUSA

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