Mitochondrial Transmembrane Proton Electrochemical Potential, Di- and Tricarboxylate Distribution and the Poise of the Malate-Aspartate Cycle in the Intact Myocardium
The unconditional requirement of closed membrane structures for oxidative energy conversion and conservation in eucaryotes entails the problem of subcellular compartmentation. Although much can be deduced from the in vitro characteristics of the permeability properties of the mitochondrial membranes and the behaviour of enzyme systems in vitro, understanding of the regulation of certain fundamental regulatory phenomena such as mitochondrial respiration has been awaiting data obtained in intact cells and tissues. Methodological progress in the field of metabolic compartmentation has brought into focus the subcellular distribution of effectors and the metabolites related to the tricarboxylic acid cycle.
KeywordsMitochondrial Membrane Mitochondrial Membrane Potential Tricarboxylic Acid Cycle Diffusion Potential Equilibrium Network
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- 1.E. A. Newsholme and C. Start, “Regulation in Metabolism”, Wiley, London (1973).Google Scholar
- 3.S. Cheema-Dhadli, B. H. Robinson, and M. L. Halperin, Proper ties of the citrate transporter in rat heart: implications for regulation of glycolysis by cytosolic citrate, Can. J. Biochem. 54: 561 (1975).Google Scholar
- 20.K. F. Lalloue and A. C. Schoolwerth, Metabolite transport in mitochondria, Ann. Rev. Biochem. 48: 87 (1979).Google Scholar
- 23.L. Hue, Role of fructose 2,6-bisphosphate in the regulation of glycolysis, Biochem. Soc. Transact. 11: 246 (1983).Google Scholar
- 24.H. A. Krebs and R. L. Veech, Pyridine nucleotide interrela tions, in: “The Energy Level and Metabolic Control in Mitochondria”, S. Papa, J. M. Tager, E. Quagliariello, and E. C. Slater, ed., Adriatica Editrice, Bari (1969).Google Scholar
- 25.P. V. Vignais, Molecular and physiological aspects of adenine nucleotide transport in mitochondria, Biochim. Biophys. Acta 496: 1 (1976).Google Scholar