Heart Myocytes as Models of the Cellular Response to Ischemia
Isolated adult heart cells (myocytes) with properties suitable for metabolic studies are now available in a number of laboratories. These cells are Ca2+-tolerant and show excellent viability and stability. They also retain the electrical properties, responsiveness to hormones and the complex morphological features of heart muscle cells in situ (1–5). Such myocyte preparations should be of considerable value in defining the response of heart cells to ischemia. Myocytes offer a unique opportunity to examine metabolic properties and transport reactions in ventricular muscle cells in the absence of variables due to tissue perfusion and without contributions from vascular cells or other cell types that may be present in intact tissue. In addition, the myocytes can be subjected to uniform perturbations in media of well-defined composition, can be serially sampled to determine the time course of responses, and are amenable to controlled disruption for compartmental analysis (6,7).
KeywordsHeart Cell Phasic Contraction Anaerobic Incubation Calcium Paradox Contraction Band Necrosis
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