The Oxygen Dependence of Cellular Energy Metabolism

  • David F. Wilson
  • Maria Erecińska
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 194)


It is well known that supply of oxygen to the heart is equal to the rate of oxygen utilization over a wide range of physical work loads (see Eckenhoff et al., 1947; Alella et al., 1955; Neely et al., 1967; Nuutinen et al., 1982). This precise regulation of oxygen delivery is expressed in the fact that the arterial venous difference in oxygen tension remains essentially constant when the heart work rates increase from minimal to near maximal levels. However, such precise regulation requires a tissue “oxygen sensor” i.e. an oxygen dependent metabolic system which detects changes in tissue oxygen tension in the physiological range and transduces this information into a form which regulates vascular resistance. In the present communication we will summarize data which indicate that the oxygen sensor for regulation of coronary flow is mitochondrial oxidative phosphorylation. We will then demonstrate that this metabolic pathway is oxygen sensitive in the physiological range of oxygen tensions both in vivo and in vitro and thus fulfills the requirements for the tissue “oxygen sensor”.


Oxygen Tension Coronary Flow Oxygen Sensor Mitochondrial Oxidative Phosphorylation Tissue Oxygen Tension 
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  1. Alella, A., Williams, F.L., Bolene-Williams, C. and Katz, L.N., 1955, Interrelation between cardiac oxygen consumption and coronary blood flow, Am. J. Physiol., 183: 570–582.Google Scholar
  2. Aw, T.Y. and Jones, D.P. 1982, Secondary bioenergetic hypoxia: inhibition of sulfation and glucuronidation reactions in isolated hepatocytes at low 02 concentration, J. Biol. Chem., 257; 8997–9004.PubMedGoogle Scholar
  3. Eckenhoff, J.E., Hafkenschiel, J.H., Laudmesser, C.M. and Harmel, M.,1947, Cardiac oxygen metabolism and control of the coronary circulation, Am. J. Physiol., 149: 634–649.PubMedGoogle Scholar
  4. Erecidska, M., Wilson, D.F. and Nishiki, K., 1978, Homeostatic regulation of cellular energy metabolism: Experimental characterization in vivo and fit to a model., Amer. J. Physiol., 234: C82 - C89.Google Scholar
  5. Holian, A., Owen, C.S. and Wilson, D.F., 1977, Control of respiration in isolated mitochondria: Quantitative evaluation of the dependence of respiratory rates on [ATP],[ADP] and [Pi], Arch. Biochem. Biophys., 181: 164–171.PubMedCrossRefGoogle Scholar
  6. Jones, D.P. and Kennedy, F.G., 1982, Intracellular 02 gradients in cardiac myocytes. Lack of a role for myoglobin in facilitation of intracellular 02 diffusion, Biochem. Biophys. Res. Commun., 105: 419–424.PubMedCrossRefGoogle Scholar
  7. Jones, D.P., and Mason, H.S., 1978, Gradients of 02 concentration in hepatocytes, J. Biol. Chem. 253: 4874–4880.PubMedGoogle Scholar
  8. Kashiwagura, T., Wilson, D.F. and Erecidska, M., 1984, Oxygen dependence of cellular metabolism: The effect of 02 tension on gluconeogenesis and urea synthesis in isolated rat hepatocytes, J. Cell. Physiol. in Press.Google Scholar
  9. Longmuir, I.S., 1957, Respiration rate of rat liver cells at low oxygen concentrations, Biochem. J. 65: 378–382.Google Scholar
  10. Neely, J.R., Liebmeister, H., Battersby, E.J. and Morgan, H.E., 1967, Effect of pressure development on oxygen consumption by isolated rat heart, Amer. J. Physiol. 212: 804–814.PubMedGoogle Scholar
  11. Nuutinen, E.M., Nishiki, K., Erecitiska, M. and Wilson, D.F., 1982, Role of mitochondrial oxidative phosphorylation in regulation of coronary blood flow, Am. J. Physiol., 243: H159 - H169.PubMedGoogle Scholar
  12. Oshino, N., Sugano, T., Oshino, R. and Chance, B., 1974, Mitochondrial function under hypoxic conditions: The steady states of cytochromes a + a and their relation to mitochondrial energy states, Biochim. Biophys. Acta, 368: 298–310.PubMedCrossRefGoogle Scholar
  13. Peterson, L.C., Nicholls, P. and Degn, H., 1974, The effect of energization on the apparent Michaelis-Menten constant for oxygen in mitochondrial respiration, Biochem.J. 142: 249–252.Google Scholar
  14. Reyes, J.G., Erdmann, W., Mardis, M., Karp, R.B., King, M. and Lell, W.A., 1978, Evidence for existence of intramyocardial steal, in “Oxygen transport to Tissue III”, I.A. Silver, M. Erecidska and H. Bicher eds. Plenum, New York, p. 755–760.Google Scholar
  15. Sugano, T., Oshino, N.and Chance, B., 1974, Mitochondrial functions under hypoxic conditions: The steady states of cytochrome c reduction and of energy metabolism. Biochem. Biophys. Acta, 347: 340–358.Google Scholar
  16. Warburg, O. and Kubowitz, F., 1929, Atmung bei sehr kleinen Sauerstoffdrucken, Biochem. Z., 214: 5–18.Google Scholar
  17. Wilson, D.F., Owen, C.S. and Erecidska, M., Drown, C., and Silver, I.A., 1979a, The oxygen dependence of cellular energy metabolism, Arch. Biochem. Biophys., 195: 485–493.Google Scholar
  18. Wilson, D.F., Erecinska, M., Drown, C. and Silver, I.A., 1977a, Effect of oxygen tension on cellular energetics, Amer. J. Physiol., 233 (5): C135 - C140.Google Scholar
  19. Wilson, D.F., Owen, C.S. and Erecifiska, M., 1979b, Quantitative dependence of mitochondrial oxidative phosphorylation on oxygen concentration: A mathematical model., Arch. Biochem. Biophys., 195: 494–504.PubMedCrossRefGoogle Scholar
  20. Wilson, D.F., Owen, C.S. and Holian, A., 1977b, Control of mitochondrial respiration: A quantitative evaluation of the roles of cytochrome c and oxygen, Arch. Biochem. Biophys., 182: 749–762.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • David F. Wilson
    • 1
  • Maria Erecińska
    • 1
  1. 1.Department of Biochemistry an BiophysicsUniversity of PennsylvaniaPhiladelphiaUSA

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