Differential Regulation of Specific Gene Expression in Mouse Kidney by Androgens and Antiandrogens

  • James F. Catterall
  • Cheryl S. Watson
  • Kimmo K. Kontula
  • Olli A. Janne
  • C. Wayne Bardin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 196)


Androgens, as other steroid hormones, interact with a receptor protein to regulate gene expression in target tissues.1 Much of the work to date on this subject has involved analysis of the abundant gene products stimulated by androgens in the rat prostate2,3 and seminal vesicle.4–6 However, no attempts were made in these studies to correlate specific gene expression with androgen receptor dynamics or to compare the androgen sensitivity of these different genes in male accessory sex organs.


Androgen Receptor Ornithine Decarboxylase Mouse Kidney mRNA Concentration Receptor Concentration 
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  1. 1.
    C. W. Bardin and J. F. Catterall, Testosterone: A major determinant of extragenital sexual dimorphism. Science 211: 1285 (1981).PubMedCrossRefGoogle Scholar
  2. 2.
    M. J. Page and M. G. Parker, Androgen-regulated rat prostatic C3 gene transfected in mouse mammary tumor cells. Cell 32: 495 (1983).PubMedCrossRefGoogle Scholar
  3. 3.
    J. G. Dodd, P. C. Sheppard, R. J. Matusik, Characterization and cloning of rat dorsal prostate mRNAs. J. Biol. Chem. 258: 10731 (1983).PubMedGoogle Scholar
  4. 4.
    P.-E. Mansson, E. Sugino, S. E. Harris, Use of cloned double stranded cDNA coding for a major androgen dependent protein in rat seminal vesicle secretion: the effect of testosterone in gene expression. Nucleic Acids Res. 9: 935 (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    J. C. Kandala, M. K. Kistler, R. P. Lawther, W. S. Kistler, Characterization of a genomic clone for rat seminal vesicle secretory protein IV. Nucleic Acids Res. 11: 3169 (1983).PubMedCrossRefGoogle Scholar
  6. 6.
    D. McDonald, L. Williams, P. McTurk, F. Fuller, E. McIntosh, S. Higgins, Isolation and characterization of genes for androgen-responsive secretory proteins of rat seminal vesicles. Nucleic Acids Res. 11: 917 (1983).PubMedCrossRefGoogle Scholar
  7. 7.
    J. J. Toole, N. D. Hastie, W. A. Held, An abundant androgen-regulated mRNA in the mouse kidney. Cell 17: 441 (1979).PubMedCrossRefGoogle Scholar
  8. 8.
    C. S. Watson, D. Salomon, J. F. Catterall, A Structure and expression of androgen-regulated genes in mouse kidney. Ann. NY Acad. Sci. 438: 101 (1984).CrossRefGoogle Scholar
  9. 9.
    F. G. Berger, K. W. Gross, G. Watson, Isolation and characterization of a DNA sequence complementary to an androgen-inducible messenger RNA from mouse kidney. J. Biol. Chem. 256: 7006 (1981).PubMedGoogle Scholar
  10. 10.
    K. K. Kontula, T. K. Torkkeli, C. W. Bardin, 0. A. Janne, Androgen induction of ornithine decarboxylase mRNA in mouse kidney as studied by complementary DNA. Proc. Natl. Acad. Sci. USA 81: 731 (1984).CrossRefGoogle Scholar
  11. 11.
    L. McConlogue, M. Gupta, L. Wu, P. Coffino, Molecular cloning and expression of the mouse ornithine decarboxylase gene. Proc Natl. Acad. Sci. USA 81: 540.Google Scholar
  12. 12.
    J. F. Catterall, S. L. Leary, Detection of early changes in androgen-induced mouse renal ß-glucuronidase messenger ribonucleic acid using cloned complementary deoxyribonucleic acid. Biochemistry 22: 6049 (1983).PubMedCrossRefGoogle Scholar
  13. 13.
    R. Palmer, P. M. Gallagher, W. L. Boyko, R. E. Ganschow, Genetic control of levels of murine kidney glucuronidase mRNA in response to androgen. Proc. Natl. Acad. Sci. USA 80: 7596 (1983).PubMedCrossRefGoogle Scholar
  14. 14.
    L. P. Bullock and C. W. Bardin, Androgen receptors and testicular feminization. J. Clin. Endocrinol. Metab. 35: 955 (1972).CrossRefGoogle Scholar
  15. 15.
    C. W. Bardin, L. P. Bullock, R. J. Sherins, I. Mowszowicz, W. R. Blackburn, Androgen metabolism and mechanism of action in male pseudohermaphrodism: A study of testicular feminization. Recent Prog. Horm. Res. 29: 65 (1973).Google Scholar
  16. 16.
    K. Paigen, Acid hydrolases as models of genetic control. Ann. Rev. Genet. 13: 417 (1979).PubMedCrossRefGoogle Scholar
  17. 17.
    L. P. Bullock, Androgen and progestin stimulation of ornithine decarboxylase activity in the mouse kidney. Endocrinology 112: 1903 (1983).PubMedCrossRefGoogle Scholar
  18. 18.
    V. Isomaa, A. E. I. Pajunen, C. W. Bardin, 0. A. Janne, Nuclear androgen receptors in the mouse kidney: Validation of a new assay. Endocrinology 111: 833 (1982).Google Scholar
  19. 19.
    R. Neri, K. Florence, P. Koziol, S. van Cleave, A biological profile of a nonsteroidal antiandrogen, SCH 13521 (4’-nitro3’-trifluoromethylisobutyranilide). Endocrinology 91: 427 (1972).PubMedCrossRefGoogle Scholar
  20. 20.
    W. Wray, P. M. Conn, V. P. Wray, Isolation of nuclei using hexylene glycol. Meths. Cell. Biol. 14: 69 (1977).CrossRefGoogle Scholar
  21. 21.
    J. A. Cidlowski, J. W. Thanassi, Extraction of nuclear glucocorticoid receptor complexes with pyridoxal 5’-phosphate. Biochem. Biophys. Res. Commun. 82: 1140 (1978).PubMedCrossRefGoogle Scholar
  22. 22.
    A. E. I. Pajunen, V. V. Isomaa, 0. A. Janne, C. W. Bardin, Androgenic regulation of ornithine decarboxylase activity in mouse kidney and its relationship to changes in cytosol and nuclear androgen receptor concentration. J. Biol. Chem. 257: 8190 (1982).PubMedGoogle Scholar
  23. 23.
    B. Attardi, S. Ohno, Cytosol androgen receptor from kidney of normal and testicular feminized (Tfm) mice. Cell 2: 205 (1974).PubMedCrossRefGoogle Scholar
  24. 24.
    U. Gehring, G. M. Tomkins, Characterization of a hormone receptor defect in the androgen-insensitivity mutant. Cell 3: 59 (1974).PubMedCrossRefGoogle Scholar
  25. 25.
    T. 0. Fox, Androgen-and estrogen-binding macromolecules in developing mouse brain: Biochemical and genetic evidence. Proc. Natl. Acad. Sci. USA 72: 4304 (1975).Google Scholar
  26. 26.
    R. T. Swank, K. Paigen, R. Davey, V. Chapman, C. Labarca, G. Watson, R. Ganschow, E. J. Brandt, E. Novak, Genetic regulation of mammalian glucuronidase. Recent Prog. Horm. Res. 34: 401 (1978).Google Scholar
  27. 27.
    G. Watson, R. A. Davey, C. Labarca, K. Paigen, Genetic determination of kinetic parameters in ß-glucuronidase induction by androgen. J. Biol. Chem. 256: 3005 (1981).PubMedGoogle Scholar
  28. 28.
    G. M. Lawson, B. J. Knoll, C. J. March, S. L. C. Woo, M.-J. Tsai, B. W. O’Malley, Definition of the 5’ and 3’ structural boundaries of the chromatin domain containing the ovalbumin multigene family. J. Biol. Chem. 257: 1501 (1982).PubMedGoogle Scholar
  29. 29.
    K. S. Bloom, J. N. Anderson, Hormonal regulation of the conformation of the ovalbumin gene in chick oviduct chromatin. J. Biol. Chem. 257: 13018 (1982).PubMedGoogle Scholar
  30. 30.
    R. D. Palmiter, E. R. Mulvihill, J. H. Shephard, G. S. McKnight, Steroid hormone regulation of ovalbumin and conalbumin gene transcription. J. Biol. Chem. 256: 7910 (1981).PubMedGoogle Scholar
  31. 31.
    T. 0. Fox, S. J. Wieland, Isoelectric focusing of androgen receptors from wild-type and Tfm mouse kidneys. Endocrinology 109: 790 (1981).Google Scholar
  32. 32.
    K. K. Kontula, P. J. Seppanen, P. Van Duyne, C. W. Bardin, 0. A. Janne, Effect of a nonsteroidal antiandrogen, flutamide, on androgen receptor dynamics and ornithine decarboxylase gene expression in mouse kidney. Endocrinology 116: 226 (1985).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • James F. Catterall
    • 1
  • Cheryl S. Watson
    • 1
  • Kimmo K. Kontula
    • 1
  • Olli A. Janne
    • 1
  • C. Wayne Bardin
    • 1
  1. 1.The Population CouncilNew YorkUSA

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