Microphotometry for Determining the Reaction Rate of O2 and CO with Red Blood Cells in the Chorioallantoic Capillary
The combination rate of O2 and CO with the erythrocyte is one of the crucial determinants for assessing the gas exchange in the lung, the placenta and the chorioallantois. A stopped flow or rapid flow reaction apparatus has well been employed to measure the reaction rate of O2 and CO with the red cell or hemoglobin solution. These flow methods have, however, some limitations in determining the reaction velocity as follows: 1) It is a difficult task to measure the reaction rate at a low Pco level comparable with that required for measurement of the pulmonary diffusing capacity for CO. 2) Pco in red cell suspension cannot be maintained constant throughout the reaction. The reduction of Pco becomes serious especially in a suspension with low Pco. 3) The apparatus requires a lot of blood. 4) In addition to a routine process, a microscopic examination is often required to ensure that no significant crenation or hemolysis occurs.
KeywordsChicken Embryo Chorioallantoic Membrane Silicon Membrane Diffusion Velocity Hemoglobin Solution
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