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Microphotometry for Determining the Reaction Rate of O2 and CO with Red Blood Cells in the Chorioallantoic Capillary

  • Masaji Mochizuki
  • Hiroshi Tazawa
  • Tsukasa Ono
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 191)

Abstract

The combination rate of O2 and CO with the erythrocyte is one of the crucial determinants for assessing the gas exchange in the lung, the placenta and the chorioallantois. A stopped flow or rapid flow reaction apparatus has well been employed to measure the reaction rate of O2 and CO with the red cell or hemoglobin solution. These flow methods have, however, some limitations in determining the reaction velocity as follows: 1) It is a difficult task to measure the reaction rate at a low Pco level comparable with that required for measurement of the pulmonary diffusing capacity for CO. 2) Pco in red cell suspension cannot be maintained constant throughout the reaction. The reduction of Pco becomes serious especially in a suspension with low Pco. 3) The apparatus requires a lot of blood. 4) In addition to a routine process, a microscopic examination is often required to ensure that no significant crenation or hemolysis occurs.

Keywords

Chicken Embryo Chorioallantoic Membrane Silicon Membrane Diffusion Velocity Hemoglobin Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1).
    Fukui, K and Y. Kakiuchi: Jap. J. Physiol. 20, 332–347, 1970.CrossRefGoogle Scholar
  2. 2).
    Fukui, K and M. Mochizuki: Mono. Series Res. Inst. Appl. Electr. No.20, 69–78, 1972.Google Scholar
  3. 3).
    Sirs, J. A. and F. J. W. Roughton: J. Appl. Physiol. 18, 158–165, 1963.PubMedGoogle Scholar
  4. 4).
    Tazawa, H.: Respir. Physiol. 13, 160–170, 352–300, 1971.PubMedCrossRefGoogle Scholar
  5. 4a).
    Tazawa, H.: Respir. Physiol. 17, 21–31, 1973.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • Masaji Mochizuki
    • 1
  • Hiroshi Tazawa
    • 1
  • Tsukasa Ono
    • 1
  1. 1.Research Institute of Applied ElectricityHokkaido UniversitySapporoJapan

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