Abstract
The exchange of information between a cell and its environment is frequently mediated by ionic movements through the plasma membrane. The physiology of sea urchin sperm is a representative case in which this phenomenon occurs. For instance, when these cells are spawned into sea water, their motility and respiration activate (Ohtake, 1976; Nishioka and Cross, 1978; Christen et al., 1982). This activation is mostly dependent on the transport of Na+ and H+ through the plasma membrane (Nishioka and Cross, 1978). The sperm acrosome reaction, a prerequisite for egg fertilization, is also modulated by ionic fluxes (Schakmann et al., 1978). This reaction in sea urchin sperm consists of the exocytosis of the acrosomal vesicle located at the anterior region of the sperm head (Dan, 1952; Summers et al., 1975) and leads to the exposure of a protein required for sperm-egg binding (Vacquier and Moy, 1977; Glabe and Lennarz, 1979) and of lytic enzymes that digest the coat of the egg (Levine and Walsh, 1979; Green and Summers, 1980). Also during the process, the intracellular cyclic nucleotides increase (Garbers and Kopf, 1980), and an actin-containing acrosomal tubule is formed (Tilney et al., 1973).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Blatz, A. L., and Magleby, K. L., 1983, Single voltage-dependent chloride-selective channels of large conductance in cultured rat muscle, Biophys. J. 43:237–240.
Christen, R., Schackmann, R. W., and Shapiro, B. M., 1982, Elevation of intracellular pH activates respiration and motility of sperm of the sea urchin, Strongylocentrotus pur-puratus, J. Biol. Chem. 257:14881–14890.
Collins, F., and Epel, D., 1977, The role of calcium ions in the acrosome reaction of sea urchin sperm, Exp. Cell Res. 106:211–222.
Coronado, R., and Latorre, R., 1983, Phospholipid bilayers made from monolayers on patch-clamp pipettes, Biophys. J. 43:231–236.
Cross, N., 1983, Isolation and electrophoretic characterization of the plasma membrane of seaurchin sperm, J. Cell Sci. 59:13–25.
Dan, J., 1952, Studies on the acrosome. I. Reaction to egg water and other stimuli, Biol. Bull. 103:54–66.
Dan, J., 1954, Studies on the acrosome. II. Effect of calcium deficiency, Biol. Bull. 107:335–349.
Darszon, A., 1983, Strategies in the reassembly of membrane proteins into lipid bilayer systems and their functional assay, J. Bioenerg. Biomembr. 15:321–334.
Darszon, A., Gould, M., de De la Torre, L., and Vargas, I., 1984a, Response of isolated sperm plasma membranes from sea urchins to egg jelly, Eur. J. Biochem. 144:515–522.
Darszon, A., Liévano, A., and Sánchez, J., 1984b, Single channel recording from bilayer derived from isolated sea urchin plasma membranes, Biophys. J. 45:308a.
Decker, G. L., Joseph, D. B., and Lennarz, W. J., 1976, A study of factors involved in induction of the acrosome reaction in sperm of the sea urchin Arbacia punctulata, Dev. Biol. 53:115–125.
Epel, D., 1978, Mechanisms of activation of sperm and egg during fertilization of sea urchin gametes, Curr. Top. Dev. Biol. 12:185–246.
Ferry, D. R., and Glossmann, H., 1982, Identification of putative calcium channels in skeletal muscle microsomes, FEBS Lett. 148:331–337.
Garbers, D. L., and Kopf, G. S., 1980, The regulation of spermatozoa by calcium and cyclic nucleotides, Adv. Cyclic Nucleotide Res. 13:251–306.
Garcfa-Soto, J., and Darszon, A., 1984, Effect of Ca2+- antagonists on the Ca2+ uptake associated with the acrosome reaction of sea urchin sperm, Biophys. J. 45:40a.
Garcia-Soto, J., and Darszon, A., 1985, High pH-induced acrosome reaction and Ca2+ uptake in sea urchin sperm suspended in Na+-free sea water, Dev. Biol. 110:338–345.
Garcia-Soto, J., de De la Torre, L., Vargas, I., and Darszon, A., 1984, Response of isolated sea urchin sperm head plasma membranes to egg jelly, in 8th International Biophysics Congress (Bristol, U.K.), I.U.P.A.B. Abstracts, p. 289.
Glabe, C. G., and Lennarz, W. J., 1979, Species-specific sperm adhesion in sea urchins. A quantitative investigation of binding-mediated egg agglutination, J. Cell Biol. 83:595–604.
Gould, R. J., Murphy, K. M. M., and Snyder, S. H., 1982, [3H]Nitrendipine-labeled calcium channels discriminated inorganic calcium agonists and antagonists, Proc. Natl. Acad. Sci. U.S.A. 79:3656–3660.
Green, J. D., and Summers, R. G., 1980, Ultrastructural demonstration of trypsin-like protease in acrosome of sea urchin sperm, Science 209:398–400.
Hamill, O. P., Marty, A., Neher, E., Sackmann, B., and Sigworth, F., 1981, Improved patch-clamp techniques for high resolution recordings from cells and cell-free membrane patches, Pfluegers Arch. 391:85–100.
Wilmsen, U., Methfessel, C., Hanke, W., and Boheim, G., 1983, Channel current fluctuations studies with solvent-free bilayers using Neher-Sackmann pipettes, in Physical Chemistry of Transmembrane Ion Motions (G. Spach, ed.), Elsevier/North-Holland, Amsterdam pp. 479–485.
Jacobson, B. S., 1977, Isolation of plasma membrane from eukaryotic cells on polylysine-coated Polyacrylamide beads, Biochim. Biophys. Acta 471:331–335.
Kohlhardt, M., and Fleckenstein, A., 1977, The inhibition of the slow sodium inward current by nifedipine in mammalian ventricular myocardium, Naunyn Schmiedebergs Arch. Pharmacol. 298:267–272.
Kopf, G. S., and Garbers, D. L., 1980, Calcium and fucose-sulfate rich polymer regulate sperm cyclic nucleotide metabolism and the acrosome reaction, Biol. Reprod. 22:1118–1126.
Lopo, A. C., and Vacquier, V. D., 1980, Antibody to sperm surface glycoprotein inhibits the egg jelly-induced acrosome reaction of sea urchin sperm, Dev. Biol. 79:325–333.
Latorre, R., and Miller, C., 1983, Conduction and selectivity in potassium channels, J. Membr. Biol. 71:11–30.
Lee, U.C., Johnson, C., and Epel, D., 1983, Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm, Dev. Biol. 95:31–45.
Levine, A. E., and Walsh, K. A., 1979, Involvement of an acrosin-like enzyme in the acrosome reaction of sea urchin sperm, Dev. Biol. 72:126–137.
Liévano, A., Sanchez, J., and Darszon, A., 1985, Single channel activity of bilayers derived from sea urchin sperm plasma membranes formed at the tip of a patch electrode, Dev. Biol. 112:253–257.
Nishioka, D., and Cross, N., 1978, The role of external Na+ in sea urchin fertilization, in Cell Reproduction (E. R. Dirksen, D. Prescott, and C. F. Fox, eds.), Academic Press, New York, pp. 403–413.
Ohtake, H., 1976, Respiratory behaviour of sea-urchin spermatozoa. Effect of pH and egg water on the respiratory rate, J. Exp. Zool. 198:303–312.
Plotek, Y., and Atlas, D., 1983, Characterization of benextramine as an irreversible α-adrenergic blocker and as a blocker of potassium-activated calcium channels, Eur. J. Biochem. 133:539–544.
Schackmann, R. W., and Shapiro, B. M., 1981, A partial sequence of ionic changes associated with the acrosome reaction of Strongylocentrotus purpuratus, Dev. Biol. 81:145–154.
Schackmann, R. W., Eddy, E. M., and Shapiro, B. M., 1978, The acrosome reaction of Strongylocentrotus purpuratus sperm. Ion requirements and movements, Dev. Biol. 65:483–495.
Schackmann, R. W., Christen, R., and Shapiro, B. M., 1981, Membrane potential depolarization an increased intracellular pH accompanying the acrosome reaction of sea urchin sperm, Proc. Natl. Acad. Sci. U.S.A. 78:6066–6070.
Schindler, H. G., 1979, Exchange and interactions between lipid layers at the surface of a liposome solutions, Biochim. Biophys. Acta 555:316–336.
Schindler, H. G., and Rosenbush, J., 1978, Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers, Proc. Natl. Acad. Sci. U.S.A. 75:3751–3755.
Schwarz, W., Neumke, B., and Palade, P. T., 1981, K-current fluctuations in inward-rectifying channels of frog skeletal muscle, J. Membr. Biol. 63:85–92.
SeGall, G. K., and Lennarz, W. J., 1979, Chemical characterization of the component of the egg jelly coat from sea urchin eggs responsible for induction of the acrosome reaction, Dev. Biol. 71:33–48.
SeGall, G. K., and Lennarz, W. J., 1981, Jelly coat and induction of the acrosome reaction in echinoid sperm, Dev. Biol. 86:87–93.
Stanfield, P. R., 1983, Tetraethylammonium ions and the potassium permeability of excitable cells, Rev. Physiol. Biochem. Pharmacol. 97:1–67.
Suárez-Isla, B., Wan, K., Lindstrom, J., and Montai, M., 1983, Single-channel recordings from purified acetylcholine receptors reconstituted in bilayers formed at the tip of patch pippets, Biochemistry 22:2319–2323.
Summers, R. G., Hylander, B. L., Colwin, L. H., and Colwin, A. L., 1975, The functional anatomy of the echinoderm spermatozoon and its interaction with the egg at fertilization, Am.Zool. 15:523–551.
Tan, K. N., and Tashjian, A. J., Jr., 1984, Voltage-dependent calcium channels in pituitary cells in culture. I. Characterization by 45Ca2+ fluxes, J. Biol. Chem. 259:418–426.
Tilney, L. G., Hatano, S., Ishikawa, H., and Mooseker, M. S., 1973, The polymerization of actin: Its role in the generation of the acrosomal process of certain echinoderm sperm, J. Cell Biol. 59:109–126.
Ulbricht, W., Wagner, H. H., and Schmidtmayer, J., 1982, Effects of aminopyridines on potassium currents of the model membrane, in Aminopyridines and Similarly Acting Drugs: Effects on Nerves, Muscles and Synapses. (Lechat, P. et al., eds.) Pergamon, Oxford, pp. 29–41.
Vacquier, V. D., and Moy, G. W., 1977, Isolation of binding: The protein responsible for adhesion of sperm to sea urchin eggs, Proc. Natl. Acad. Sci. U.S.A. 74:2456–2460.
Wilmsen, U., Methfessel, C., Hanke, W., and Boheim, G., 1982, in Phisico Chimie des Mouvements Ioniques Jransmembranaires, Ecole Nationale Superieure de Chimie, Paris, p. 50.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Darszon, A., García-Soto, J., Liévano, A., Sánchez, J.A., Islas-Trejo, A.D. (1986). Ionic Channels in the Plasma Membrane of Sea Urchin Sperm. In: Latorre, R. (eds) Ionic Channels in Cells and Model Systems. Series of the Centro de Estudios Científicos de Santiago. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5077-4_19
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5077-4_19
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5079-8
Online ISBN: 978-1-4684-5077-4
eBook Packages: Springer Book Archive