Channels in Kidney Epithelial Cells
Since the first conclusive demonstration of the presence of channels in epithelia, in particular the demonstration of amiloride-sensitive sodium channels involved in active Na+ reabsorption across the apical membrane of the frog skin (Lindemann and Van Driessche, 1976), the question arose as to how much of the conductive properties of epithelia are controlled by ion channels. The advent of the patch-clamp technique allowed a new way of investigating this problem in epithelial cells from kidney. Thus, by use of the patch-clamp technique on isolated kidney tubules and on cultured kidney cell lines, a comparison can be made between the conductance properties of the membranes and the properties of the ion channels found in the same segments. We can then begin to assess what components of the conductive properties of these cells are accounted for by channel activity.
KeywordsApical Membrane Chromaffin Cell Apical Cell Membrane Frog Skin Distal Nephron
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