Optical Detection of ATP Release from Stimulated Endocrine Cells

A Universal Marker of Exocytotic Secretion of Hormones
  • Eduardo Rojas
  • Rosa Maria Santos
  • Andres Stutzin
  • Harvey B. Pollard
Part of the Series of the Centro de Estudios Científicos de Santiago book series (SCEC)


In many types of secretory cells the secretory granules and vesicles contain adenosine-5’-triphosphate (ATP) in addition to the specific transmitters or hormones. Examples include chromaffin cells (Winkler and Westhead, 1980), pancreatic B cells (Sussman and Leitner, 1977), nerve cells (Dowdall et al., 1974; Wagner et al., 1978; Pollard and Pappas, 1979), platelets (Code, 1952; Born, 1958; Weil-Malherbe and Bone, 1958), and mast cells (Uvnas, 1971). It should therefore be possible, at least in principle, to obtain quantitative, real-time measurements of the kinetics of secretion of vesicle contents by monitoring the ATP release (Leitner et al., 1975; Ito et al., 1980; Livett and Boksa, 1984) using the luciferase-catalyzed luminescent oxidation of luciferin.


Endocrine Cell Chromaffin Cell Krebs Solution Catecholamine Release Chromaffin Granule 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Eduardo Rojas
    • 1
  • Rosa Maria Santos
    • 1
  • Andres Stutzin
    • 1
  • Harvey B. Pollard
    • 1
  1. 1.Laboratory of Cell Biology and Genetics, NIADDKNational Institutes of HealthBethesdaUSA

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